Human TMPRSS2 ELISA Kit (ab283552)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 4.266 pg/ml
- Range: 15.625 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human TMPRSS2 ELISA Kit
See all TMPRSS2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 4.9% Inter-assay Sample n Mean SD CV% Extract 3 5.8% -
Sample type
Cell culture supernatant, Urine, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
4.266 pg/ml -
Range
15.625 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 104 96% - 112% Serum 91 83% - 96% Hep Plasma 94 89% - 100% EDTA Plasma 92 87% - 95% Cit plasma 95 93% - 102% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human TMPRSS2 ELISA kit (ab283552) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human TMRPSS2 protein in human serum, urine, cell culture supernatants, cell culture extract and tissue culture extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TMPRSS2 with 4.27 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Human TMPRSS2 is a plasma membrane-anchored serine protease that participates in proteolytic cascades involving physiological functions of the prostate. TMPRSS2 is understood to facilitate human coronaviruses SARS-CoV and SARS-CoV-2 infections via two independent mechanisms; Proteolytic cleavage of ACE2 receptor which promotes viral uptake, and cleavage of coronavirus spike glycoproteins which activates the glycoprotein for host cell entry. TMPRSS2 also proteolytically cleaves and activates human coronavirus EMC (MERS), and the fusion glycoproteins F0 of Sendai virus, human metapneumovirus, human parainfluenza 1, 2, 3, 4a and 4b viruses. TMPRSS2 is essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9) and is involved in proteolytic cleavage and activation of hemagglutinin protein which is essential for viral infectivity. The antibodies in this product were raised to a portion of the extracellular region excluding the peptidase domain. The standard protein in this product is approximately 30% of full length TMPRSS2.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human TMPRSS2 Capture Antibody 1 x 600µl 10X Human TMPRSS2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human TMPRSS2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Tissue specificity
Expressed strongly in small intestine. Also expressed in prostate, colon, stomach and salivary gland. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 LDL-receptor class A domain.
Contains 1 peptidase S1 domain.
Contains 1 SRCR domain. -
Cellular localization
Cell membrane and Secreted. Activated by cleavage and secreted. - Information by UniProt
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Alternative names
- D16Ertd61e
- Epitheliasin
- FLJ41954
see all -
Database links
- Entrez Gene: 7113 Human
- Omim: 602060 Human
- SwissProt: O15393 Human
- Unigene: 439309 Human
Images
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Human TMPRSS2 concentration was interpolated from the TMPRSS2 standard curve.
Human TMPRSS2 concentration was interpolated from the TMPRSS2 standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human TMPRSS2 ELISA Kit (ab283552). Wild-type and TMPRSS2 knockout LNCaP (ab273745) cells were assessed in duplicate (n=2). Data are represented as the mean and error bars represent standard deviation.
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SimpleStep ELISA Protocol DiagramSimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Recombinant Antibody BenefitsTo learn more about the advantages of recombinant antibodies see here.
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Example of human TMPRSS2 standard curve in Sample Diluent NS.The TMPRSS2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed
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Interpolated concentrations of spike TMPRSS2 in serum, plasma and cell culture supernatant samples.The concentrations of TMPRSS2 were measured in duplicates and interpolated from the TMPRSS2 standard curves. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, plasma (heparin) 50%. The interpolated values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of spike TMPRSS2 in 50% urine and 100% cell culture supernatant samplesThe concentrations of TMPRSS2 were measured in duplicate and interpolated from the TMPRSS2 standard curves. The interpolated values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native TMPRSS2 in human lung tissue extract and LNCaP cell extract based on 2,000 µg/mL and 300 µg/ml extract loads, respectively.The concentrations of TMPRSS2 were measured in duplicate and interpolated from the TMPRSS2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TMPRSS2 concentration was determined to be 166.86 pg/ml in human lung tissue extract and 909.11 pg/mL in LNCaP cell extract.
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100% Urine from 4 bladder cancer patients was measured in duplicate.Interpolated values are plotted (mean +/- SD, n+2). The mean native TMPRSS2 concentration was determined to be 20.27 pg/ml. *Undetected.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab283552 has not yet been referenced specifically in any publications.