Human Transferrin ELISA Kit (ab187391)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.39 ng/ml
- Range: 0.63 ng/ml - 40 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Transferrin ELISA Kit
See all Transferrin kits
Intra-assay Sample n Mean SD CV% Overall 8 5.2% Inter-assay Sample n Mean SD CV% Overall 3 8.3%
Sample typeCell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma
Assay typeSandwich (quantitative)
Range0.63 ng/ml - 40 ng/ml
Sample specific recovery Sample type Average % Range Serum 92 86% - 104% Cell culture media 101 97% - 103% Hep Plasma 114 112% - 116% EDTA Plasma 107 95% - 115% Cit plasma 115 104% - 121%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
Human Transferrin ELISA Kit (ab187391) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Transferrin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Transferrin with 0.39 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY This kit recognizes both native and recombinant human Transferrin protein in serum, plasma, and cell culture supernatant samples only. Urine, saliva, and cell/tissue extract samples have not been tested with this kit.
CROSS REACTIVITY Human albumin, ferritin, and hemoglobin were prepared at 40 ng/mL and assayed for cross reactivity. No cross-reactivity was observed.
SPECIES REACTIVITY This kit recognizes human Transferrin protein.
Other species reactivity was determined by measuring 1:50,000 serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution. Reactivity < 3% was determined for the following species: Mouse, Rat, Cow
Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Human Transferrin Capture Antibody 1 x 600µl 10X Human Transferrin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Transferrin Lyophilized Purified Protein (ab91435) 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 2 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
Tissue specificityExpressed by the liver and secreted in plasma.
Involvement in diseaseDefects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
Sequence similaritiesBelongs to the transferrin family.
Contains 2 transferrin-like domains.
- Information by UniProt
- Beta 1 metal binding globulin
- Beta-1 metal-binding globulin
- Entrez Gene: 7018 Human
- Omim: 190000 Human
- SwissProt: P02787 Human
- Unigene: 518267 Human
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Standard Curve comparison between the original Human Transferrin SimpleStep ELISA kit and current Human Transferrin SimpleStep ELISA kit.
The Transferrin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Example of human Transferrin standard curve in Sample Diluent NS. The Transferrin standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of Transferrin were measured in duplicates, interpolated from the Transferrin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:50,000, plasma (EDTA) 1:50,000, plasma (citrate) 1:50,000 and plasma (heparin) 1:50,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Transferrin concentration was determined to be 1.82 mg/mL in serum, 1.84 ng/mL in plasma (EDTA) and 1.86 mg/mL in plasma (citrate) and 1.43 ng/mL in plasma (heparin).
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Transferrin concentration was determined to be 2.38 mg/mL with a range of 1.54 – 3.2 mg/mL.
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Transferrin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
ab187391 has been referenced in 4 publications.
- Harder NHO et al. Effects of Dietary Glucose and Fructose on Copper, Iron, and Zinc Metabolism Parameters in Humans. Nutrients 12:N/A (2020). PubMed: 32854403
- Vatine GD et al. Human iPSC-Derived Blood-Brain Barrier Chips Enable Disease Modeling and Personalized Medicine Applications. Cell Stem Cell 24:995-1005.e6 (2019). PubMed: 31173718
- Allden SJ et al. The Transferrin Receptor CD71 Delineates Functionally Distinct Airway Macrophage Subsets during Idiopathic Pulmonary Fibrosis. Am J Respir Crit Care Med N/A:N/A (2019). PubMed: 31051082
- Anantpadma M et al. Large Scale Screening and Identification of Novel Ebolavirus and Marburgvirus Entry Inhibitors. Antimicrob Agents Chemother N/A:N/A (2016). Sandwich ELISA ; Human . PubMed: 27161622