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Human Urokinase type Plasminogen Activator ELISA Kit (ab108917)

Submit a review Q&A (3)References (4)
Typical Standard Curve

    Key features and details

    • Sensitivity: 0.2 ng/ml
    • Range: 0.25 ng/ml - 8 ng/ml
    • Sample type: Cell culture supernatant, Plasma, Serum, Tissue, Urine
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    Overview

    • Product name

      Human Urokinase type Plasminogen Activator ELISA Kit
      See all Urokinase kits

    • Detection method

      Colorimetric

    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall 4.9%
      Inter-assay
      Sample n Mean SD CV%
      Overall 7.2%

    • Sample type

      Cell culture supernatant, Urine, Serum, Plasma, Tissue

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      = 0.2 ng/ml

    • Range

      0.25 ng/ml - 8 ng/ml

    • Recovery

      98.5 %

    • Assay time

      4h 00m

    • Assay duration

      Multiple steps standard assay

    • Species reactivity

      Reacts with: Human

    • Product overview

      Abcam’s Urokinase type Plasminogen Activator Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Urokinase type Plasminogen Activator (uPA) in plasma, serum, urine, milk, cell culture supernatants and tissue extracts.


      A Urokinase type Plasminogen Activator specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Urokinase type Plasminogen Activator specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Urokinase type Plasminogen Activator captured in plate.


      The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

    • Platform

      Microplate

    Properties

    Images

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108917.

    References (4)

    Publishing research using ab108917? Please let us know so that we can cite the reference in this datasheet.

    ab108917 has been referenced in 4 publications.

    • Zhou H  et al. Synergistic inhibitory effects of an engineered antibody-like molecule ATF-Fc and trastuzumab on tumor growth and invasion in a human breast cancer xenograft mouse model. Oncol Lett 14:5189-5196 (2017). PubMed: 29113154
    • Andersen H  et al. Diabetic nephropathy is associated with increased urine excretion of proteases plasmin, prostasin and urokinase and activation of amiloride-sensitive current in collecting duct cells. Nephrol Dial Transplant 30:781-9 (2015). Sandwich ELISA ; Human . PubMed: 25609736
    • Stæhr M  et al. Aberrant glomerular filtration of urokinase-type plasminogen activator in nephrotic syndrome leads to amiloride-sensitive plasminogen activation in urine. Am J Physiol Renal Physiol 309:F235-41 (2015). Sandwich ELISA ; Human . PubMed: 25972510
    • Liu SJ  et al. Berberine suppresses in vitro migration of human aortic smooth muscle cells through the inhibitions of MMP-2/9, u-PA, AP-1, and NF-?B. BMB Rep 47:388-92 (2014). PubMed: 24286329

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    Can you please tell me whether the format of this assay is ONE 96 well plate, meaning that all reactions should be used simultaneously, or if the format is 12 strips of 8, implying that experiments can be run on multiple days?

    Also, with regards to these two kits, from what I can tell I should have no problem detecting tPA and uPA from cell culture supernatants or cell homogenates?

    Read More
    Answer



    Both ab108914 and ab108917 contain 12, 8 well strips.


    You are correct that you should be able to detect tPA and uPA from cell culture supernatants or cell homogenates with both these kits.

    Read More

    Question

    How much tissueand lysis buffer volume is needed for this kit.

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    Answer

    Thank you for your email.

    We generally recommend our customer using 400-500ul of lysis buffer per 5-10 mg of tissues however you may have to adjust it based upon your results.

    500ul lysis buffer for 1 million cells would be ideal.

    I hope this information will be helpful.

    Read More

    Question

    How much tissueand lysis buffer volume is needed for this kit.

    Read More
    Answer

    Thank you for your email.

    We generally recommend our customer using 400-500ul of lysis buffer per 5-10 mg of tissues however you may have to adjust it based upon your results.

    500ul lysis buffer for 1 million cells would be ideal.

    I hope this information will be helpful.

    Read More

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