Human VEGF Receptor 2 ELISA Kit (ab100665)
Key features and details
- Sensitivity: 70 pg/ml
- Range: 34.3 pg/ml - 25000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human VEGF Receptor 2 ELISA Kit -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 70 pg/ml -
Range
34.3 pg/ml - 25000 pg/ml -
Recovery
95 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 96.23 86% - 104% Serum 96.46 85% - 105% Plasma 94.78 84% - 103% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s VEGF Receptor 2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF Receptor 2 in serum, plasma and cell culture supernatants. (Genecards: KDR)
This assay employs an antibody specific for Human VEGF Receptor 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF Receptor 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VEGF Receptor 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF Receptor 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimization may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human VEGF Receptor 2 2 vials Recombinant Human VEGF Receptor 2 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml VEGF Receptor 2 Microplate (12 x 8 wells) 1 unit -
Research areas
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Relevance
VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
Cellular localization
Cell Membrane, Cytoplasmic, Nuclear and Secreted -
Alternative names
- CD309
- Fetal liver kinase 1
- FLK1
see all -
Database links
- Entrez Gene: 3791 Human
- Omim: 191306 Human
- SwissProt: P35968 Human
Images
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Typical standard curve
Representative standard curve using ab100665
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Typical standard curveRepresentative standard curve using ab100665
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab100665 has been referenced in 2 publications.
- Oldak L et al. Two Biosensors for the Determination of VEGF-R2 in Plasma by Array SPRi. Molecules 28:N/A (2022). PubMed: 36615347
- Kinoshita Y et al. Mead acid inhibits the growth of KPL-1 human breast cancer cells in vitro and in vivo. Oncol Rep 32:1385-94 (2014). PubMed: 25109488