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    products/elisa/human-vimentin-profiling-elisa-kit-ab173190.pdf

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Human Vimentin Profiling ELISA Kit (ab173190)

  • Datasheet
  • SDS
  • Protocol Booklet
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Typical Standard Curve
  • Experiment showing total Vimentin levels in various cell lines
  • Vimentin protein expression level in the analyzed cell lysates

Key features and details

  • Sensitivity: 8 µg/ml
  • Sample type: Adherent cells, Suspension cells, Tissue Homogenate, Tissue Lysate
  • Detection method: Colorimetric
  • Assay type: Quantitative
  • Reacts with: Human

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Overview

  • Product name

    Human Vimentin Profiling ELISA Kit
    See all Vimentin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 3 7.5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 12%
  • Sample type

    Adherent cells, Suspension cells, Tissue Homogenate, Tissue Lysate
  • Assay type

    Quantitative
  • Sensitivity

    8 µg/ml
  • Recovery

    > 80 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture media 80 71% - 87%
    Fetal Bovine Serum 89 81% - 93%
    Bovine Serum Albumin 89 83% - 84%
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Vimentin Human Profiling in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of total vimentin protein in Human samples.


    The assay employs an antibody specific to Vimentin coated onto well plate strips. Samples are pipetted into the wells and Vimentin present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti-Vimentin detector antibody is added. After washing away unbound detector antibody, HRP-conjugated label specific for the detector antibody is pipetted into the wells. The wells are again washed, a TMB development solution is added to the wells and blue color develops in proportion to the amount of bound Vimentin.

  • Notes

    Vimentin is a type III intermediate filament (IF) protein that forms the cytoskeletal framework in the cytoplasm of eukaryotic cells. Other structural proteins such as actin and tubulin are highly conserved in different cell types, whereas IF proteins are expressed in a highly tissue specific manner. Due to vimentin being the major cytoskeletal protein in mesenchymal cells, it is used as a marker of mesenchymally-derived cells or cells undergoing epithelial-to-mesenchymal transition (EMT) during normal development and metastatic progression.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    10X Blocking Buffer 1 x 6ml
    10X HRP Label 1 x 1ml
    10X Vimentin Detector Antibody 1 x 1ml
    10X Wash Buffer 1 x 40ml
    Extraction Buffer 1 x 15ml
    HeLa Whole Cell RIPA Extract 1 x 300µl
    HRP Development Solution 1 x 12ml
    Vimentin Microplate 1 unit
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class III
    • Vimentin
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Cancer
    • Tumor biomarkers
    • Other
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Neuroscience
    • Development
    • Neuroscience
    • Processes
  • Function

    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • Tissue specificity

    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • Involvement in disease

    Cataract 30
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Domain

    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • Post-translational
    modifications

    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P08670 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • CTRCT30
    • Epididymis luminal protein 113
    • FLJ36605
    • HEL113
    • VIM
    • VIME_HUMAN
    • Vimentin
    see all
  • Database links

    • Entrez Gene: 7431 Human
    • Omim: 193060 Human
    • SwissProt: P08670 Human
    • Unigene: 455493 Human
    • Unigene: 691131 Human

    Images

    • Typical Standard Curve
      Typical Standard Curve

      Typical standard curve generated when using the Vimentin Human Profiling ELISA kit.

    • Experiment showing total Vimentin levels in various cell lines
      Experiment showing total Vimentin levels in various cell lines

      HeLa cell lysate, Jurkat cell lysate, SH SY5Y cell lysate, MCF7 cell lysate (not shown) and MDA-MB-231 cell lysate were tested using this kit. HeLa cell lysate shown as standard control sample, varying concentration other cell Lysates were analyzed within working range of the assay.

    • Vimentin protein expression level in the analyzed cell lysates
      Vimentin protein expression level in the analyzed cell lysates

      Western blot of cell lysates (top panel): 1) HeLa, 2) Jurkat, 3) Sy5y, 4) MCF7, 5) MDA-MB-231 (10 μg/lane) using primary antibodies: rabbit anti-vimentin (1/1000 dilution) and mouse anti-vimentin (ab8978, 1/1000 dilution); Secondary antibodies used were goat anti‑rabbit 680-RD (Red, 1/10,000) and goat anti‑mouse 800 (Green, 1/10,000). Blot was scanned using a LI­­‑COR® Odyssey® imager and overlay shows target specificity for total vimentin (yellow). Bar graph (bottom panel) represents relative mOD/min (600 nm) observed for 10 μg/mL cell lysate, except for MDA-MB-231 cell lysate which reports mOD/min (600 nm) for 1 μg/mL.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab173190? Please let us know so that we can cite the reference in this datasheet.

    ab173190 has been referenced in 1 publication.

    • Jung S  et al. Influence of Implant Material and Surface on Mode and Strength of Cell/Matrix Attachment of Human Adipose Derived Stromal Cell. Int J Mol Sci 21:N/A (2020). PubMed: 32526920

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