Human Vimentin Profiling ELISA Kit (ab173190)
Key features and details
- Sensitivity: 8 µg/ml
- Sample type: Adherent cells, Suspension cells, Tissue Homogenate, Tissue Lysate
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Human
Product nameHuman Vimentin Profiling ELISA Kit
See all Vimentin kits
Intra-assay Sample n Mean SD CV% Overall 3 7.5% Inter-assay Sample n Mean SD CV% Overall 3 12%
Sample typeAdherent cells, Suspension cells, Tissue Homogenate, Tissue Lysate
> 80 %
Sample specific recovery Sample type Average % Range Cell culture media 80 71% - 87% Fetal Bovine Serum 89 81% - 93% Bovine Serum Albumin 89 83% - 84%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s Vimentin Human Profiling in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of total vimentin protein in Human samples.
The assay employs an antibody specific to Vimentin coated onto well plate strips. Samples are pipetted into the wells and Vimentin present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti-Vimentin detector antibody is added. After washing away unbound detector antibody, HRP-conjugated label specific for the detector antibody is pipetted into the wells. The wells are again washed, a TMB development solution is added to the wells and blue color develops in proportion to the amount of bound Vimentin.
Vimentin is a type III intermediate filament (IF) protein that forms the cytoskeletal framework in the cytoplasm of eukaryotic cells. Other structural proteins such as actin and tubulin are highly conserved in different cell types, whereas IF proteins are expressed in a highly tissue specific manner. Due to vimentin being the major cytoskeletal protein in mesenchymal cells, it is used as a marker of mesenchymally-derived cells or cells undergoing epithelial-to-mesenchymal transition (EMT) during normal development and metastatic progression.
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Storage instructionsPlease refer to protocols.
Components 1 x 96 tests 10X Blocking Buffer 1 x 6ml 10X HRP Label 1 x 1ml 10X Vimentin Detector Antibody 1 x 1ml 10X Wash Buffer 1 x 40ml Extraction Buffer 1 x 15ml HeLa Whole Cell RIPA Extract 1 x 300µl HRP Development Solution 1 x 12ml Vimentin Microplate 1 unit
FunctionVimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Tissue specificityHighly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Involvement in diseaseCataract 30
Sequence similaritiesBelongs to the intermediate filament family.
DomainThe central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
modificationsFilament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
- Information by UniProt
- Epididymis luminal protein 113
- Entrez Gene: 7431 Human
- Omim: 193060 Human
- SwissProt: P08670 Human
- Unigene: 455493 Human
- Unigene: 691131 Human
Typical standard curve generated when using the Vimentin Human Profiling ELISA kit.
HeLa cell lysate, Jurkat cell lysate, SH SY5Y cell lysate, MCF7 cell lysate (not shown) and MDA-MB-231 cell lysate were tested using this kit. HeLa cell lysate shown as standard control sample, varying concentration other cell Lysates were analyzed within working range of the assay.
Western blot of cell lysates (top panel): 1) HeLa, 2) Jurkat, 3) Sy5y, 4) MCF7, 5) MDA-MB-231 (10 μg/lane) using primary antibodies: rabbit anti-vimentin (1/1000 dilution) and mouse anti-vimentin (ab8978, 1/1000 dilution); Secondary antibodies used were goat anti‑rabbit 680-RD (Red, 1/10,000) and goat anti‑mouse 800 (Green, 1/10,000). Blot was scanned using a LI‑COR® Odyssey® imager and overlay shows target specificity for total vimentin (yellow). Bar graph (bottom panel) represents relative mOD/min (600 nm) observed for 10 μg/mL cell lysate, except for MDA-MB-231 cell lysate which reports mOD/min (600 nm) for 1 μg/mL.
Datasheets and documents
ab173190 has been referenced in 1 publication.
- Jung S et al. Influence of Implant Material and Surface on Mode and Strength of Cell/Matrix Attachment of Human Adipose Derived Stromal Cell. Int J Mol Sci 21:N/A (2020). PubMed: 32526920