Human KIM1 ELISA Kit (TIM-1) (ab235081)
Key features and details
- Sensitivity: 1.279 pg/ml
- Range: 7.813 pg/ml - 500 pg/ml
- Sample type: Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman KIM1 ELISA Kit (TIM-1)
See all TIM 1 kits
Assay typeSandwich (quantitative)
Range7.813 pg/ml - 500 pg/ml
Assay time2h 0m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
The human KIM1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is a complete kit for the quantitative measurement of KIM1 protein in urine.
The ELISA kit is ultra sensitive, reliably measuring as little as 1.2 pg/ml of Kim1. This enables the use of reduced input sample and results in reduced matrix interference. It is high throughput with results in <2 hours from up to 38 samples in duplicate. The kit uses a monoclonal antibody to KIM-1 immobilized on a microtiter plate to bind the KIM-1 in the standards or sample. After a short incubation the excess sample or standard is washed out and a biotinylated monoclonal antibody to KIM-1 is added. This antibody binds to the KIM-1 captured on the plate. After a short incubation the excess antibody is washed out and Streptavidin conjugated to Horseradish peroxidase is added, which binds to the biotin on the monoclonal antibody. The plate is then incubated. Once the incubation is complete, excess conjugate is washed out and TMB substrate solution is added. An HRP-catalyzed reaction generates a blue color in the solution. Stop solution is added to stop the substrate reaction. The resulting yellow color is read at 450 nm. The amount of signal is directly proportional to the level of KIM-1 in the sample.
Kim1 is an early biomarker for kidney injury or disease. It is the most highly upregulated protein in the proximal tubule of the injured kidney. It exists in very low levels in normal kidneys but when upregulated during injury, it is detectable in urine in a wide variety of human diseases. KIM-1 is a potential biomarker for renal injury, which would suggest it has great importance in various kidney diseases and disorders such as chronic kidney disease, as well as acute tubular necrosis and acute kidney failure.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Assay Buffer 1 x 60ml KIM-1 coated microplate (12x 8 well strips) 1 unit KIM-1 Conjugate 1 x 10ml KIM-1 Detector Antibody 1 x 10ml KIM-1 Standard (25ng/mL) 1 x 0.5ml Plate Sealer 3 units Stop Solution 2 1 x 10ml TMB Substrate 1 x 10ml Wash Buffer Concentrate 1 x 100ml
FunctionMay play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
Tissue specificityWidely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
Sequence similaritiesBelongs to the immunoglobulin superfamily. TIM family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
- Information by UniProt
- HAVCR 1
- Entrez Gene: 26762 Human
- Omim: 606518 Human
- SwissProt: Q96D42 Human
- Unigene: 129711 Human
Datasheets and documents
ab235081 has been referenced in 4 publications.
- Diaz-Riera E et al. Vitamin D Binding Protein and Renal Injury in Acute Decompensated Heart Failure. Front Cardiovasc Med 9:829490 (2022). PubMed: 35757319
- Leng Y et al. Diagnostic role of urinary CA-2 in urinary stones and its prediction of complications. Am J Transl Res 14:8686-8694 (2022). PubMed: 36628213
- Li XQ et al. Critical roles of cytokine storm and secondary bacterial infection in acute kidney injury development in COVID-19: A multi-center retrospective cohort study. J Med Virol 93:6641-6652 (2021). PubMed: 34314040
- Tsai KF et al. Association between Enzyme-Linked Immunosorbent Assay-Measured Kidney Injury Markers and Urinary Cadmium Levels in Chronic Kidney Disease. J Clin Med 11:N/A (2021). PubMed: 35011897