Human S100A4 ELISA Kit (ab283547)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.41 ng/ml
- Range: 0.94 ng/ml - 60 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human S100A4 ELISA Kit
See all S100A4 kits -
Detection method
Colorimetric -
Precision
Inter-assay Sample n Mean SD CV% supernatant 3 6.8% -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.41 ng/ml -
Range
0.94 ng/ml - 60 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 94 92% - 95% Serum 99 90% - 108% EDTA Plasma 87 83% - 94% Cit plasma 86 85% - 88% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human S100A4 ELISA kit (ab283547) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human S100A4 protein in human serum, pasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human S100A4 with 0.41 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
S100A4, encoded by the S100A4 gene, is a member of the S100 calcium binding family of proteins. S100A4 is known to be highly associated with components of the cytoskeleton to regulate the motility that can serve as a metastasis factor and invasiveness of cancer cells. When the gene is upregulated, it subsequently changes the cell’s morphology, making it more susceptible to invasion from other proteins that contribute to metastasis.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human S100A4 Capture Antibody 1 x 600µl 10X Human S100A4 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human S100A4 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains. - Information by UniProt
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Alternative names
- 18A2
- 42A
- calcium Placental protein
see all -
Database links
- Entrez Gene: 6275 Human
- Omim: 114210 Human
- SwissProt: P26447 Human
- Unigene: 654444 Human
Images
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To learn more about the advantages of recombinant antibodies see here.
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The S100A4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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The concentrations of S100A4 were measured in duplicates, interpolated from the S100A4 standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (EDTA) 40% and A172 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean S100A4 concentration was determined to be 70.2 ng/mL in plasma (EDTA) and 3.95 ng/mL in A172 supernatant.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab283547 has not yet been referenced specifically in any publications.