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    products/elisa/mouse-axl-elisa-kit-ab207619.pdf

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RecombinantSimpleStep

Mouse Axl ELISA Kit (ab207619)

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Other - Mouse Axl ELISA Kit (ab207619)
  • Example of mouse Axl standard curve.
  • Interpolated concentrations of native Axl in mouse serum, plasma, and urine samples.
  • Comparison of secreted Axl in unstimulated and PMA/PHA-stimulated L929 Cells.
  • Sandwich ELISA - Mouse Axl ELISA Kit (ab207619)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 10.8 pg/ml
  • Range: 62.5 pg/ml - 4000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

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Overview

  • Product name

    Mouse Axl ELISA Kit
    See all Axl kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 2.4%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 3.4%
  • Sample type

    Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    10.8 pg/ml
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 106 101% - 114%
    Urine 109 104% - 114%
    Serum 104 91% - 116%
    Hep Plasma 83 74% - 91%
    EDTA Plasma 100 86% - 111%
    Cit plasma 102 90% - 111%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Cow, Pig
  • Product overview

    Mouse Axl ELISA Kit (ab207619) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Axl protein in cell culture supernatant, serum, urine, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Axl with 10.8 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Axl is a transmembrane receptor tyrosine kinase on the plasma membrane. The growth factor GAS6 is a ligand for Axl and regulates many physiological processes including cell survival, cell proliferation, migration and differentiation. The antibodies used in this kit were raised against a portion of the extracellular domain of Axl.

  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse Axl Capture Antibody 1 x 600µl
    10X Mouse Axl Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BR 1 x 6ml
    Mouse Axl Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Other
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Tyrosine kinases
    • Receptor tyrosine kinases
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Signal transducers
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Oncoprotein ELISA kits
  • Relevance

    The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, the Axl protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors such as vitamin K dependent protein growth arrest specific gene 6. It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. Axl is a chronic myelogenous leukemia associated oncogene and also associated with colon cancer and melanoma. It is in close vicinity to the bcl3 oncogene which is at 19q13.1-q13.2. The Axl gene is evolutionarily conserved between vertebrate species. This gene has two different alternatively spliced transcript variants (AXL1 and AXL2).
  • Alternative names

    • Adhesion related kinase
    • AI323647
    • Ark
    • AXL oncogene
    • AXL receptor tyrosine kinase
    • AXL transforming gene
    • AXL transforming sequence/gene
    • JTK11
    • Oncogene AXL
    • Tyro7
    • Tyrosine protein kinase receptor UFO
    • UFO
    see all
  • Database links

    • SwissProt: Q00993 Mouse

    Associated products

    • Alternative Versions

      • Mouse Axl Antibody Pair - BSA and Azide free (ab241675)
    • ELISA kits

      • Mouse Axl ELISA Kit (ab100669)

    Images

    • Other - Mouse Axl ELISA Kit (ab207619)
      Other - Mouse Axl ELISA Kit (ab207619)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of mouse Axl standard curve.
      Example of mouse Axl standard curve.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native Axl in mouse serum, plasma, and urine samples.
      Interpolated concentrations of native Axl in mouse serum, plasma, and urine samples.

      The concentrations of Axl were measured in duplicates, interpolated from the Axl standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 4%, plasma (citrate) 4%, plasma (EDTA) 4%, and urine 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Axl concentration was determined to be 113 ng/mL in serum, 73.3 ng/mL in plasma (citrate), 88.9 ng/mL in plasma (heparin), and 112 ng/mL in urine.

    • Comparison of secreted Axl in unstimulated and PMA/PHA-stimulated L929 Cells.
      Comparison of secreted Axl in unstimulated and PMA/PHA-stimulated L929 Cells.

      L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. Axl was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the Axl Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 169 pg/mL unstimulated, 696 pg/mL stimulated, and undetectable in media.

    • Sandwich ELISA - Mouse Axl ELISA Kit (ab207619)
      Sandwich ELISA - Mouse Axl ELISA Kit (ab207619)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab207619? Please let us know so that we can cite the reference in this datasheet.

    ab207619 has not yet been referenced specifically in any publications.

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