Mouse CXCL11 ELISA Kit (I-TAC) (ab204519)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.4 pg/ml
- Range: 7.8 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse CXCL11 ELISA Kit (I-TAC)
See all CXCL11 kits
Intra-assay Sample n Mean SD CV% Overall 5 4.8% Inter-assay Sample n Mean SD CV% Overall 3 4.7%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range7.8 pg/ml - 500 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 115 110% - 120% Serum 113 107% - 116% Hep Plasma 90 86% - 92% EDTA Plasma 104 94% - 122% Cit plasma 116 116% - 121%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Mouse
Does not react with: Sheep
Mouse CXCL11 ELISA Kit (I-TAC) (ab204519) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL11 (I-TAC) protein in cell culture supernatant, plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CXCL11 (I-TAC) with 1.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CXCL11 (I-TAC) is a chemokine for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment and in skin immune responses.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Mouse CXLC11 Capture Antibody 1 x 600µl 10X Mouse CXLC11 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse CXCL11 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
FunctionChemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.
Tissue specificityHigh levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
- Information by UniProt
- b R1
- Entrez Gene: 56066 Mouse
- SwissProt: Q9JHH5 Mouse
- Unigene: 131723 Mouse
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of CXCL11 were measured in duplicate and interpolated from the CXCL11 standard curve and corrected for sample dilution.
Heparin appears to release erythrocyte bound CXCL11, resulting in higher concentration than plasma samples collected with EDTA or sodium citrate.
To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
ab204519 has been referenced in 2 publications.
- Peltzer N et al. LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis. Nature 557:112-117 (2018). PubMed: 29695863
- Peltzer N et al. Holding RIPK1 on the Ubiquitin Leash in TNFR1 Signaling. Trends Cell Biol 26:445-461 (2016). PubMed: 26877205