Mouse Frataxin ELISA Kit (ab199078)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 5 pg/ml
- Range: 31.25 pg/ml - 2000 pg/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse Frataxin ELISA Kit
See all Frataxin kits
Intra-assay Sample n Mean SD CV% NIH 3T3 3 1.3% Inter-assay Sample n Mean SD CV% NIH 3T3 3 2%
Sample typeCell culture extracts, Tissue Extracts
Assay typeSandwich (quantitative)
Range31.25 pg/ml - 2000 pg/ml
Sample specific recovery Sample type Average % Range Serum 100 95% - 109% Fetal Bovine Serum 101 99% - 103% serum free media 101 99% - 103%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Mouse
Mouse Frataxin ELISA Kit (ab199078) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Frataxin protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Frataxin with 5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
This kit recognizes both native and recombinant mouse Frataxin protein in cell and tissue extract samples only.
Whole blood, saliva, urine, serum and plasma samples have not been tested with this kit.
This kit recognizes mouse Frataxin protein.
Human reactivity was determined to be 48%, by measuring 310 pg/mL human recombinant protein and comparing the raw O.D. values to the mouse standard protein raw O.D. values assayed at the same concentration, and expressing the ratio as a percentage of human protein reactivity to mouse protein reactivity within the assay.
Mouse Frataxin is a 23 kDa nuclear-encoded mitochondrial protein. The gene is localized on chromosome 9 and is highly conserved during evolution. The gene is expressed in every cell, although in varying levels in different tissues and during development. The specific function of frataxin is still unknown, but it has been shown to play a role in iron metabolism. Studies have demonstrated that the deletion of the frataxin gene in yeast results in iron accumulation in mitochondria and loss of respiration. Recombinant frataxin has been shown to bind iron in vitro, and increased mitochondrial iron levels have been observed in patients with Friedreich's Ataxia (FRDA). FRDA is an autosomal recessive, progressive degenerative disease characterized by neurodegeneration and cardiomyopathy it is the most common inherited ataxia.
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PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Mouse Frataxin Capture Antibody 1 x 600µl 10X Mouse Frataxin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Mouse Frataxin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
RelevanceFunction: Promotes the biosynthesis of heme and assembly and repair of iron-sulfur clusters by delivering Fe2+ to proteins involved in these pathways. May play a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe2+ to Fe3+; the oligomeric form but not the monomeric form has in vitro ferroxidase activity. May be able to store large amounts of iron in the form of a ferrihydrite mineral by oligomerization; however, the physiological relevance is unsure as reports are conflicting and the function has only been shown using heterologous overexpression systems. Modulates the RNA-binding activity of ACO1.
Cellular localizationCytoplasmic and Mitochondrial
- Entrez Gene: 14297 Mouse
- SwissProt: O35943 Mouse
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Background-subtracted data values (mean +/- SD) are graphed.
Example of Frataxin mouse standard curve. The Frataxin mouse standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
NIH 3T3, RAW264.7 and mouse liver extracts were prepared as described previously and interpolated values corrected by dilution factor (mean +/- SD) are graphed.
Interpolated values of mouse Frataxin protein are plotted for the indicated cell and tissue extract based on an extract load of 1,250 ng/mL.
Native mouse Frataxin protein was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Datasheets and documents
ab199078 has been referenced in 2 publications.
- Belbellaa B et al. High Levels of Frataxin Overexpression Lead to Mitochondrial and Cardiac Toxicity in Mouse Models. Mol Ther Methods Clin Dev 19:120-138 (2020). PubMed: 33209958
- Piguet F et al. Rapid and Complete Reversal of Sensory Ataxia by Gene Therapy in a Novel Model of Friedreich Ataxia. Mol Ther 26:1940-1952 (2018). PubMed: 29853274