Mouse Galectin 3 ELISA Kit (ab203369)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.4 pg/ml
- Range: 7.81 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Galectin 3 ELISA Kit
See all Galectin 3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 3.7% Inter-assay Sample n Mean SD CV% Serum 3 8.9% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.4 pg/ml -
Range
7.81 pg/ml - 500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 96 94% - 98% Serum 107 105% - 108% Cell culture media 94 92% - 97% Hep Plasma 96 94% - 98% EDTA Plasma 111 107% - 115% Cit plasma 108 104% - 111% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Goat, Pig -
Product overview
Mouse Galectin 3 ELISA Kit (ab203369) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Galectin 3 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Galectin 3 with 2.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Galectin-3 (Gal-3, Galactoside-binding protein, Mac-2 antigen) is in the Galectin family of carbohydrate-binding proteins and is active intracellularly and extracellularly. It is widely expressed, including by fibroblasts and macrophages. Intracellularly, Galectin-3 can function in the nucleus as a pre-mRNA splicing factor and in the cytoplasm it has roles in endocytosis, exocytosis and apoptosis. Extracellularly, Galectin-3 is pro-inflammatory and is involved in innate immunity. Galectin-3 can be monomeric or form higher order homo and hetero oligomers.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Galectin-3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR 1 x 6ml Mouse Galectin-3 Capture Antibody (Lyophilized) 1 vial Mouse Galectin-3 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis By similarity. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Probably forms homo- or heterodimers. Interacts with DMBT1. Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA. A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Contains 1 galectin domain. -
Cellular localization
Cytoplasmic, Nuclear and Secreted -
Alternative names
- 35 kDa lectin
- Carbohydrate-binding protein 35
- CBP 35
see all -
Database links
- Entrez Gene: 16854 Mouse
- SwissProt: P16110 Mouse
Associated products
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Alternative Versions
Images
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Other - Mouse Galectin 3 ELISA Kit (ab203369)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Galectin-3 standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of Galectin-3 in mouse serum, plasma (citrate), and plasma (EDTA).The concentrations of Galectin-3 were measured in duplicate and interpolated from the Galectin-3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 11.3 ng/mL in mouse serum, 13.2 ng/mL in mouse plasma (Citrate), and 12.9 ng/mL in mouse plasma (EDTA).
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Comparison of secreted Galectin-3 in unstimulated and PMA/PHA-stimulated J774A.1 Cells.J774A.1 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. Galectin-3 was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated J774A.1 and cell culture media. Measured values were interpolated from the Galectin-3 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 14 ng/mL unstimulated, 38.9 ng/mL stimulated, and undetectable in media.
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Sandwich ELISA - Mouse Galectin 3 ELISA Kit (ab203369)To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab203369 has been referenced in 3 publications.
- Petrovic I et al. Overexpression of Galectin 3 in Pancreatic ß Cells Amplifies ß-Cell Apoptosis and Islet Inflammation in Type-2 Diabetes in Mice. Front Endocrinol (Lausanne) 11:30 (2020). PubMed: 32117058
- Meena NK et al. Enzyme Replacement Therapy Can Reverse Pathogenic Cascade in Pompe Disease. Mol Ther Methods Clin Dev 18:199-214 (2020). PubMed: 32671132
- Di Gregoli K et al. Galectin-3 Identifies a Subset of Macrophages With a Potential Beneficial Role in Atherosclerosis. Arterioscler Thromb Vasc Biol 40:1491-1509 (2020). PubMed: 32295421