Mouse GDF-15 Matched Antibody Pair Kit (ab220131)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 7.8 pg/ml - 500 pg/ml
Overview
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Product name
Mouse GDF-15 Matched Antibody Pair Kit
See all GDF15 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
2.1 pg/ml -
Range
7.8 pg/ml - 500 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse GDF-15 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse GDF-15.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab216947. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 5 x 96 tests Mouse GDF-15 Capture Antibody 1 x 50µg Mouse GDF-15 Detector Antibody 1 x 12.5µg Mouse GDF-15 Lyophilized Protein 1 vial -
Research areas
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Tissue specificity
Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- GDF 15
- GDF-15
- Gdf15
see all -
Database links
- Entrez Gene: 23886 Mouse
- SwissProt: Q9Z0J7 Mouse
- Unigene: 31325 Mouse
Associated products
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Alternative Versions
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Related Products
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SimpleStep ELISA kits
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
Images
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Mouse GDF-15 standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse GDF-15 Matched Antibody Pair Kit (ab220131)To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab220131 has not yet been referenced specifically in any publications.