Mouse IgG ELISA Kit (ab151276)
Key features and details
- Sensitivity: 1 ng/ml
- Sample type: Cell culture media, Mouse IgG, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse, Rat
Overview
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Product name
Mouse IgG ELISA Kit
See all IgG kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% overall 3 3.3% Inter-assay Sample n Mean SD CV% overall 4 2.3% -
Sample type
Serum, Cell culture media, Mouse IgG -
Assay type
Sandwich (quantitative) -
Sensitivity
1 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 89 % - % Cell culture media 85 % - % Goat Serum 96 % - % -
Assay time
3h 15m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse, Rat
Does not react with: Goat, Cow -
Product overview
ab151276, an IgG mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IgG in mouse serum, plasma and supernatant from cell cultures.
This assay employs a mouse IgG specific antibody coated onto plate strips. Standards and samples are pipetted into the wells and IgG present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an HRP-conjugated anti-mouse IgG detector antibody is added. After washing away unbound detector antibody, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IgG bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped with the Stop Solution which changes the color from blue to yellow and the intensity can be measured at 450 nm. -
Notes
There are five classes of mammalian immunoglobulins: IgA, IgD, IgE, IgM, and IgG. IgG is the most abundant immunoglobulin and is equally distributed in blood and tissue. In mice, the IgG class is further divided into four subclasses: IgG1, IgG2a/ IgG2c (strain specific), IgG2b, and IgG3. The general immunoglobulin structure is composed of four polypeptide chains, two heavy and two light chains linked together and to each other by disulfide bonds, creating a tetrameric quaternary structure. The resulting tetramer creates two identical halves which together form a Y like structure. While the amino-terminal portions that exhibits highly variable amino-acid composition are involved in antigen binding, the C terminal constant parts are involved in complement binding, placental passage and binding to cell membrane. IgG is involved in response to a foreign antigen. The presence of IgG usually signifies a mature antibody response. IgG has a molecular weight of about 150 kDa, it can bind to many pathogens and also plays an important role in antibody-dependent cell-mediated cytotoxicity. Typically mouse serum and plasma samples contain about 7 to 10 mg/ml of IgG.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Blocking Buffer 1 x 6ml 10X GAM (H+L)-HRP Detector Antibody 1 x 1ml 20X Buffer 1 x 20ml HRP Development Solution 1 x 6ml IgG Mouse Microplate 1 unit Normal Mouse IgG Standard (Lyophilized) 1 x 1µg Stop Solution 1 x 12ml -
Research areas
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Cellular localization
Secreted -
Alternative names
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
see all
Images
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Example of Mouse IgG standard curve in 1X Incubation Buffer.
Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example dilution seriesTop image: Example dilution series of normal mouse serum (NMS) in the working range of the assay. Bottom image: Example dilution series of a mouse hybridoma cell culture media (TCM) in the working range of the assay. -
Demonstration of the tested species specificity. -
Demonstration of the component requirement test.
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab151276 has been referenced in 9 publications.
- Che N et al. Adiponectin Enhances B-Cell Proliferation and Differentiation via Activation of Akt1/STAT3 and Exacerbates Collagen-Induced Arthritis. Front Immunol 12:626310 (2021). PubMed: 33815378
- Huang Y et al. Xiaochaihu decorction relieves liver fibrosis caused by Schistosoma japonicum infection via the HSP47/TGF-ß pathway. Parasit Vectors 13:254 (2020). PubMed: 32410640
- Hao L et al. Combinational PRR Agonists in Liposomal Adjuvant Enhances Immunogenicity and Protective Efficacy in a Tuberculosis Subunit Vaccine. Front Immunol 11:575504 (2020). PubMed: 33117374
- Lee GJ et al. Angioimmunoblastic T-cell lymphoma-like lymphadenopathy in mice transgenic for human RHOA with p.Gly17Val mutation. Oncoimmunology 9:1746553 (2020). PubMed: 32923110
- Ullah N et al. Differential Immunogenicity and Protective Efficacy Elicited by MTO- and DMT-Adjuvanted CMFO Subunit Vaccines against Mycobacterium tuberculosis Infection. J Immunol Res 2020:2083793 (2020). PubMed: 32953889
- Smith B et al. Generation of two high affinity anti-mouse FcRn antibodies: Inhibition of IgG recycling in wild type mice and effect in a mouse model of immune thrombocytopenia. Int Immunopharmacol 66:362-365 (2019). PubMed: 30529500
- Ma J et al. A Multistage Subunit Vaccine Effectively Protects Mice Against Primary Progressive Tuberculosis, Latency and Reactivation. EBioMedicine 22:143-154 (2017). PubMed: 28711483
- Li J et al. Dramatic enhancement of the detection limits of bioassays via ultrafast deposition of polydopamine. Nat Biomed Eng 1:N/A (2017). ELISA ; Human . PubMed: 29082104
- Damgaard RB et al. The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity. Cell 166:1215-1230.e20 (2016). PubMed: 27523608