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  1. Link

    products/elisa/mouse-il-1-beta-elisa-kit-ab197742.pdf

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Immunology Innate Immunity Cytokines Interleukins
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RecombinantSimpleStep

Mouse IL-1 beta ELISA Kit (ab197742)

  • Datasheet
  • SDS
  • Protocol Booklet
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Other - Mouse IL-1 beta ELISA Kit (ab197742)
  • Mouse IL-1 beta standard curve comparison data
  • Example of IL-1 beta standard curve in Sample Diluent NS.
  • Example of mouse IL-1 beta standard curve in Sample Diluent NS.
  • Comparison of secreted IL-1 beta in unstimulated and LPS stimulated RAW264.7 cells.
  • Interpolated concentrations of native IL-1 beta in mouse LPS-stimulated RAW 264.7 extract based on a 50 µg/mL extract load.
  • Example of IL-1 beta standard curve in 1X Cell Extraction Buffer PTR.
  • Example of mouse IL-1 beta standard curve in 1X Cell Extraction Buffer PTR.
  • Demonstration of the linearity of dilution by the titration of RAW264.7 stimulated for 48 hours with LPS undiluted to 32-fold dilution in Sample Diluent NS.
  • Linearity of dilution in Sample Diluent NS.
  • Linearity of dilution in 1X Cell Extraction Buffer PTR.
  • Linearity of dilution in Sample Diluent NS (spiked samples)
  • Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 1 pg/ml
  • Range: 1.56 pg/ml - 100 pg/ml
  • Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, Serum, Tissue Extracts, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

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Overview

  • Product name

    Mouse IL-1 beta ELISA Kit
    See all IL-1 beta kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5 6.1%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 7.7%
  • Sample type

    Cell culture supernatant, Urine, Serum, Cell culture extracts, Tissue Extracts, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1 pg/ml
  • Range

    1.56 pg/ml - 100 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 105 100% - 108%
    Urine 94 91% - 95%
    Serum 101 98% - 103%
    Plasma 89 78% - 95%
    Tissue Homogenate 97 95% - 100%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Sheep, Cow
  • Product overview

    Mouse IL-1 beta ELISA Kit (ab197742) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-1 beta protein in cell culture extracts, cell culture supernatant, cit plasma, serum, tissue extracts, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-1 beta with 1 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.


    ASSAY SPECIFICITY


    This kit recognizes both native and recombinant mouse IL-1 beta protein in serum, plasma, and cell culture supernatant, cell and tissue extract samples only.


    Mouse brain, kidney, liver and lung homogenates were tested but no signal was detected.


    Plasma (EDTA) and plasma (heparin) samples have not been tested with this kit.


    CROSS REACTIVITY


    Recombinant Human IL-1 beta, Mouse IL-1 alpha, and Mouse IL-1 receptor1 were prepared at 5 ng/mL and assayed for cross reactivity. No cross-reactivity was observed.


    INTERFERENCE


    Recombinant Human IL-1 beta, Mouse IL-1 alpha, and Mouse IL-1 receptor1 were prepared at 5 ng/mL and tested for interference. No interference with was observed.


    SPECIES REACTIVITY


    This kit recognizes mouse IL-1 beta protein.


    No signal was observed in 25% serum samples from the following species:Human, Dog, Rabbit, Hamster

  • Notes

    IL-1 beta (IL-1β) is a 17.5 kDa cytokine protein of the Interleukin 1 family. IL-1β is secreted by macrophages and other cell types in response to inflammatory agents or infection. IL-1β plays a role in a number of cellular processes, including immune responses, bone remodeling, apoptosis and inflammatory pain hypersensitivity.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse IL-1 beta Capture Antibody 1 x 600µl
    10X Mouse IL-1 beta Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BI 1 x 6ml
    Mouse IL-1 beta Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interleukins
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cytokines and cytokine receptors ELISA kits
    • Metabolism
    • Types of disease
    • Obesity
    • Neuroscience
    • Processes
  • Function

    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
  • Tissue specificity

    Expressed in activated monocytes/macrophages (at protein level).
  • Sequence similarities

    Belongs to the IL-1 family.
  • Post-translational
    modifications

    Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
  • Cellular localization

    Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive.
  • Target information above from: UniProt accession P01584 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Catabolin
    • H1
    • IFN beta inducing factor
    • IL 1
    • IL 1 beta
    • IL-1 beta
    • IL1
    • IL1 BETA
    • IL1B
    • IL1B_HUMAN
    • IL1F2
    • Interleukin 1 beta
    • Interleukin 1 beta precursor
    • interleukin 1, beta
    • Interleukin-1 beta
    • OAF
    • Osteoclast activating factor
    • OTTHUMP00000162031
    • Preinterleukin 1 beta
    • Preinterleukin beta
    • Pro interleukin 1 beta
    see all
  • Database links

    • Entrez Gene: 16176 Mouse
    • SwissProt: P10749 Mouse
    • Unigene: 222830 Mouse

    Associated products

    • Alternative Versions

      • Mouse IL-1beta Antibody Pair - BSA and Azide free (ab241673)
    • ELISA kits

      • Mouse IL-1 beta ELISA Kit (ab100704)
      • Mouse IL-1 beta ELISA Kit (ab100705)
      • Pig IL-1 beta ELISA Kit (ab100754)
      • Mouse IL-1 beta ELISA kit (ab108866)
    • Matched antibody pairs

      • Mouse IL-1 beta Matched Antibody Pair Kit (ab210895)

    Images

    • Other - Mouse IL-1 beta ELISA Kit (ab197742)
      Other - Mouse IL-1 beta ELISA Kit (ab197742)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Mouse IL-1 beta standard curve comparison data
      Mouse IL-1 beta standard curve comparison data

      Standard curve comparison between mouse IL-1 beta SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 4-fold increase in sensitivity.

    • Example of IL-1 beta standard curve in Sample Diluent NS.
      Example of IL-1 beta standard curve in Sample Diluent NS.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Example of mouse IL-1 beta standard curve in Sample Diluent NS.
      Example of mouse IL-1 beta standard curve in Sample Diluent NS.

      The IL-1 beta standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Comparison of secreted IL-1 beta in unstimulated and LPS stimulated RAW264.7 cells.
      Comparison of secreted IL-1 beta in unstimulated and LPS stimulated RAW264.7 cells.

      RAW264.7 cells were grown in the absence (unstimulated) or presence of 5 µg/mL Lipopolysaccharide (LPS) (stimulated) for 48 hours. IL-1 beta was measured in 2-fold diluted cell culture supernatants of unstimulated and LPS stimulated RAW264.7 and cell culture media. Measured values were interpolated from the IL-1 beta Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 1.1 pg/mL unstimulated, 83.5 pg/mL stimulated. There was no detectable signal in media.

    • Interpolated concentrations of native IL-1 beta in mouse LPS-stimulated RAW 264.7 extract based on a 50 µg/mL extract load.
      Interpolated concentrations of native IL-1 beta in mouse LPS-stimulated RAW 264.7 extract based on a 50 µg/mL extract load.

      The concentrations of IL-1 beta were measured in duplicate and interpolated from the IL-1 beta standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1 beta concentration was determined to be 70 pg/mL in LPS stimulated RAW 264.7 Lysate. 

    • Example of IL-1 beta standard curve in 1X Cell Extraction Buffer PTR.
      Example of IL-1 beta standard curve in 1X Cell Extraction Buffer PTR.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Example of mouse IL-1 beta standard curve in 1X Cell Extraction Buffer PTR.
      Example of mouse IL-1 beta standard curve in 1X Cell Extraction Buffer PTR.

      The IL-1 beta standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Demonstration of the linearity of dilution by the titration of RAW264.7 stimulated for 48 hours with LPS undiluted to 32-fold dilution in Sample Diluent NS.
      Demonstration of the linearity of dilution by the titration of RAW264.7 stimulated for 48 hours with LPS undiluted to 32-fold dilution in Sample Diluent NS.

      Background-subtracted data values (mean +/- SD, n = 2) are graphed.

    • Linearity of dilution in Sample Diluent NS.
      Linearity of dilution in Sample Diluent NS.

      Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

      Native IL-1 beta was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

    • Linearity of dilution in 1X Cell Extraction Buffer PTR.
      Linearity of dilution in 1X Cell Extraction Buffer PTR.

      Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

      Native IL-1 beta was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in 1X Cell Extraction Buffer PTR.

    • Linearity of dilution in Sample Diluent NS (spiked samples)
      Linearity of dilution in Sample Diluent NS (spiked samples)

      Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

      Recombinant IL-1 beta was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.

      25% pooled serum, plasma (Citrate), and urine samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay.

       

       

    • Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)
      Sandwich ELISA - Mouse IL-1 beta ELISA Kit (ab197742)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (54)

    Publishing research using ab197742? Please let us know so that we can cite the reference in this datasheet.

    ab197742 has been referenced in 54 publications.

    • Liu H  et al. Catalytically potent and selective clusterzymes for modulation of neuroinflammation through single-atom substitutions. Nat Commun 12:114 (2021). PubMed: 33414464
    • Ponomarenko AI  et al. N-docosahexaenoylethanolamine reduces neuroinflammation and cognitive impairment after mild traumatic brain injury in rats. Sci Rep 11:756 (2021). PubMed: 33436960
    • Li Y  et al. microRNA-103a-3p confers protection against lipopolysaccharide-induced sepsis and consequent multiple organ dysfunction syndrome by targeting HMGB1. Infect Genet Evol 89:104681 (2021). PubMed: 33333289
    • Ding L  et al. Interleukin-1ß Suppresses Gastrin via Primary Cilia and Induces Antral Hyperplasia. Cell Mol Gastroenterol Hepatol 11:1251-1266 (2021). PubMed: 33347972
    • Jabir MS  et al. Green synthesis of silver nanoparticles from Eriobotrya japonica extract: a promising approach against cancer cells proliferation, inflammation, allergic disorders and phagocytosis induction. Artif Cells Nanomed Biotechnol 49:48-60 (2021). PubMed: 33403879
    View all Publications for this product

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Quick and easy to use IL1b Kit

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    The kit is very simple to use and the results yielded are very satisfactory. As compared to other kits, it require a shorter time to incubate.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 19 2021

    ab197742 – Mouse IL-1 beta SimpleStep ELISA  Kit

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    The kit works very well. The standard yield an excellent curve and mouse IL-1b was also picked up in biological samples, in this case bronchoalveolar lavage fluid from mice (control and after lung inflammation was induced).
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Monica Romera-Hernandez

    Verified customer

    Submitted Jul 27 2021

    IL-1 beta Simple step ELISA kit

    Good Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    Good one and very easy to use.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Sheelu Monga

    Verified customer

    Submitted Feb 06 2018

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