Mouse IL-10 ELISA Set (without plates) (ab47599)
Overview
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Product name
Mouse IL-10 ELISA Set (without plates)
See all IL-10 kits -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 20 pg/ml -
Range
31.25 pg/ml - 1000 pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s IL-10 Murine in vitro ELISA Set is designed for the quantitative measurement of Murine IL-10 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
The murine IL-10 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
This ELISA Set is intended to measure murine IL-10 in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
Recognizes both natural and recombinant Murine IL-10
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 5 x 96 tests 15 x 96 tests 10 x 96 tests Chromogen TMB Substrate Solution 2 vials 6 vials 4 vials Detection biotinylated anti-murine IL-10 antibody 1 x 0.55ml 3 x 0.55ml 2 x 0.55ml Murine IL-10 Capture antibody 1 x 0.5ml 3 x 0.5ml 2 x 0.5ml Murine IL-10 standard 5 vials 15 vials 10 vials Streptavidin-HRP 1 vial 3 vials 2 vials -
Research areas
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Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CSIF
- Cytokine synthesis inhibitory factor
- IL 10
see all -
Database links
- Entrez Gene: 16153 Mouse
- SwissProt: Q0VBJ1 Mouse
- SwissProt: P18893 Mouse
- Unigene: 874 Mouse
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47599 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| Notes |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
Images
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Murine IL-10 ELISA Set (ab47599)Murine IL-10 standard curve ranging from 31.25 to 1000 pg/mL
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab47599 has not yet been referenced specifically in any publications.