Mouse IL-13 ELISA Kit (ab100700)
Key features and details
- Sensitivity: 6 pg/ml
- Range: 8.23 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IL-13 ELISA Kit
See all IL-13 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 6 pg/ml -
Range
8.23 pg/ml - 2000 pg/ml -
Recovery
94 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 95.37 84% - 104% Serum 94.42 83% - 103% Plasma 93.56 82% - 102% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s IL-13 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-13 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse IL-13 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-13 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 120X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse IL-13 2 vials IL-13 Microplate (12 x 8 wells) 1 unit Recombinant Mouse IL-13 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages. -
Involvement in disease
Allergic rhinitis -
Sequence similarities
Belongs to the IL-4/IL-13 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Allergic rhinitis
- ALRH
- BHR 1
see all -
Database links
- Entrez Gene: 16163 Mouse
- SwissProt: P20109 Mouse
- Unigene: 1284 Mouse
Images
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Typical standard curve
Representative standard curve using ab100700
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Typical standard curveRepresentative standard curve using ab100700
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab100700 has been referenced in 2 publications.
- Song J & Wang J SIRT3 regulates bronchial epithelium apoptosis and aggravates airway inflammation in asthma. Mol Med Rep 25:N/A (2022). PubMed: 35234263
- Bacsi A et al. Down-regulation of 8-oxoguanine DNA glycosylase 1 expression in the airway epithelium ameliorates allergic lung inflammation. DNA Repair (Amst) 12:18-26 (2013). PubMed: 23127499