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    products/elisa/mouse-il-17a-elisa-kit-ab199081.pdf

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Immunology Innate Immunity Cytokines Interleukins
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RecombinantSimpleStep

Mouse IL-17A ELISA Kit (ab199081)

  • Datasheet
  • SDS
  • Protocol Booklet
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Other - Mouse IL-17A ELISA Kit (ab199081)
  • Mouse IL-17A standard curve comparison data.
  • Example of IL-17a standard curve prepared in Sample Diluent NS.
  • Example of IL-17a standard curve prepared in Sample Diluent 25BP.
  • Demonstration of the linearity of dilution of the assay.
  • PHA+PMA treatment of EL4 cells stimulates secretion of IL-17a.
  • Sandwich ELISA - Mouse IL-17A ELISA Kit (ab199081)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 0.5 pg/ml
  • Range: 6.25 pg/ml - 400 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

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Overview

  • Product name

    Mouse IL-17A ELISA Kit
    See all IL-17A kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 10.6%
  • Sample type

    Cell culture supernatant, Serum, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.5 pg/ml
  • Range

    6.25 pg/ml - 400 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 102.5 97.7% - 105.8%
    Cell culture media 91.1 82.7% - 99.7%
    Cit plasma 103.6 102.2% - 105.8%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Mouse IL-17A ELISA Kit (ab199081) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-17A protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-17A with 0.5 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    IL-17A is a pro-inflammatory cytokine that is secreted by a subset of activated T cells. It is a disulfide-linked homodimer with both glycosylated and non-glycosylated forms. IL-17A induces stromal cells to produce pro-inflammatory and hematopoietic cytokines, and also enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.

  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse IL-17a Capture Antibody 1 x 600µl
    10X Mouse IL-17a Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Mouse IL-17a Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 25BP 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Interleukin ELISA kits
    • Neuroscience
    • Processes
  • Function

    Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
  • Tissue specificity

    Restricted to activated memory T-cells.
  • Sequence similarities

    Belongs to the IL-17 family.
  • Post-translational
    modifications

    Found both in glycosylated and nonglycosylated forms.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q16552 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • CTLA 8
    • CTLA-8
    • CTLA8
    • cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein 8
    • Cytotoxic T lymphocyte associated serine esterase 8
    • Cytotoxic T-lymphocyte-associated antigen 8
    • IL 17
    • IL 17A
    • IL-17
    • IL-17A
    • IL17
    • IL17_HUMAN
    • Il17a
    • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8)
    • interleukin 17A
    • Interleukin-17A
    • interleukin17
    • Interleukin17A
    • OTTHUMP00000016597
    • OTTMUSP00000046003
    see all
  • Database links

    • Entrez Gene: 16171 Mouse
    • SwissProt: Q62386 Mouse

    Associated products

    • Alternative Versions

      • Mouse IL-17A Antibody Pair - BSA and Azide free (ab242038)

    Images

    • Other - Mouse IL-17A ELISA Kit (ab199081)
      Other - Mouse IL-17A ELISA Kit (ab199081)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Mouse IL-17A standard curve comparison data.
      Mouse IL-17A standard curve comparison data.

      Standard curve comparison between mouse IL-17A SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 9-fold increase in sensitivity.

    • Example of IL-17a standard curve prepared in Sample Diluent NS.
      Example of IL-17a standard curve prepared in Sample Diluent NS.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Example of IL-17a standard curve prepared in Sample Diluent 25BP.
      Example of IL-17a standard curve prepared in Sample Diluent 25BP.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Demonstration of the linearity of dilution of the assay.
      Demonstration of the linearity of dilution of the assay.

      Titration of PHA+PMA stimulated EL4 cell culture supernatant within the working range of the assay. EL4 cells were cultured in the presence of 1.5% phytohemagglutinin (PHA) and 10 ng/mL phorbol 12-myristate 13-acetate (PMA) for 48 hours. The cell culture supernatant was collected and measured in 2-fold dilution series. Background-subtracted data values (mean +/- SD, n = 2) are graphed.

    • PHA+PMA treatment of EL4 cells stimulates secretion of IL-17a.
      PHA+PMA treatment of EL4 cells stimulates secretion of IL-17a.

      EL4 cells were cultured in the absence or presence of 1.5% PHA and 10 ng/mL PMA for 48 hours. The cell culture supernatants were collected and their dilutions (as indicated) were measured with this kit. Interpolated concentrations of IL-17a adjusted for sample dilution are graphed in ng of IL-17a per mL of supernatant (mean +/- SD, n = 2). Note that IL-17a is not detectable in the untreated EL4 supernatant samples.

    • Sandwich ELISA - Mouse IL-17A ELISA Kit (ab199081)
      Sandwich ELISA - Mouse IL-17A ELISA Kit (ab199081)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab199081? Please let us know so that we can cite the reference in this datasheet.

    ab199081 has been referenced in 3 publications.

    • Zhou Y  et al. Puerarin improves graft bone defect through microRNA-155-3p-mediated p53/TNF-a/STAT1 signaling pathway. Int J Mol Med 46:239-251 (2020). PubMed: 32377717
    • Pang L  et al. Apigenin attenuates PM2.5-induced airway hyperresponsiveness and inflammation by down-regulating NF-?B in murine model of asthma. Int J Clin Exp Pathol 12:3700-3709 (2019). PubMed: 31933758
    • Jia CE  et al. Pendrin, an anion exchanger on lung epithelial cells, could be a novel target for lipopolysaccharide-induced acute lung injury mice. Am J Transl Res 8:981-92 (2016). PubMed: 27158384

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