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    products/elisa/mouse-il-17f-elisa-kit-ab204522.pdf

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Cardiovascular Angiogenesis Inhibitors
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RecombinantSimpleStep

Mouse IL-17F ELISA Kit (ab204522)

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  • SDS
  • Protocol Booklet
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Other - Mouse IL-17F ELISA Kit (ab204522)
  • Example of IL-17F standard curve in Sample Diluent NS.
  • Interpolated concentrations of IL-17F in mouse serum and plasma samples.
  • PMA + IL-1a treatment of mouse spleen induces secretion of IL-17F.
  • Sandwich ELISA - Mouse IL-17F ELISA Kit (ab204522)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 0.1 pg/ml
  • Range: 9.375 pg/ml - 600 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

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Overview

  • Product name

    Mouse IL-17F ELISA Kit
    See all IL-17F kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5 8.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 4.5%
  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.1 pg/ml
  • Range

    9.375 pg/ml - 600 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 118.6 114.8% - 121.8%
    Cell culture media 91.9 84.4% - 98.8%
    Hep Plasma 118.1 107.7% - 124.2%
    EDTA Plasma 116.6 112.8% - 123%
    Cit plasma 103.6 96.8% - 113.2%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Goat, Pig
  • Product overview

    Mouse IL-17F ELISA Kit (ab204522) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-17F protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-17F with 0.1 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    IL-17F is an IL-17 family member cytokine that stimulates the production of other cytokines such as IL-6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. IL-17F stimulates PBMC and T-cell proliferation. IL-17F inhibits angiogenesis. IL-17F is a glycosylated, disulfide-linked homodimer. IL-17F is also present as IL-17A/F heterodimer. IL-17F is expressed in activated, but not resting, CD4+ T-cells and activated monocytes. It is secreted.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse IL-17F Capture Antibody 1 x 600µl
    10X Mouse IL-17F Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BR 1 x 6ml
    Mouse IL-17F Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 25BP 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Inhibitors
    • Cardiovascular
    • Angiogenesis
    • Cytokines
    • Interleukin
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic inhibitory factors
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interleukins
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Interleukin ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cytokines and cytokine receptors ELISA kits
  • Function

    Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover (PubMed:11591732, PubMed:11591768, PubMed:11574464). Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis (PubMed:11591732). Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.
  • Tissue specificity

    Expressed in activated, but not resting, CD4+ T-cells and activated monocytes.
  • Involvement in disease

    Candidiasis, familial, 6
  • Sequence similarities

    Belongs to the IL-17 family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q96PD4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • CANDF6
    • Cytokine ML 1
    • Cytokine ML-1
    • IL 17F
    • IL 24
    • IL-17F
    • IL-24
    • Il17f
    • IL17F_HUMAN
    • Interleukin 17F
    • Interleukin 24
    • Interleukin-17F
    • Interleukin-24
    • ML 1
    • ML1
    • Mutant IL 17F
    • OTTHUMP00000016602
    see all
  • Database links

    • Entrez Gene: 257630 Mouse
    • SwissProt: Q7TNI7 Mouse
    • Unigene: 222807 Mouse

    Associated products

    • Alternative Versions

      • Mouse IL-17F Antibody Pair - BSA and Azide free (ab241728)

    Images

    • Other - Mouse IL-17F ELISA Kit (ab204522)
      Other - Mouse IL-17F ELISA Kit (ab204522)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of IL-17F standard curve in Sample Diluent NS.
      Example of IL-17F standard curve in Sample Diluent NS.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of IL-17F in mouse serum and plasma samples.
      Interpolated concentrations of IL-17F in mouse serum and plasma samples.

      The concentrations of IL-17F were measured in duplicates, interpolated from the IL-17F standard curve and corrected for sample dilution. Note that 1X Diluted samples were pre-diluted to 25%. The interpolated, dilution factor-corrected values are plotted in pg of IL 17F per mL of neat sample (mean +/- SD, n=2).

    • PMA + IL-1a treatment of mouse spleen induces secretion of IL-17F.
      PMA + IL-1a treatment of mouse spleen induces secretion of IL-17F.

      Mouse spleen cells were cultured in the absence or presence of 10 ng/mL PMA + 10 ng/mL Mouse recombinant IL-1a for 4 days. The cell culture supernatant samples were collected. The concentrations of IL-17F were measured in the diluted supernatants in duplicates, interpolated from the IL-17F standard curve and corrected for sample dilution. Note that 1X Diluted supernatant samples were neat. The interpolated, dilution factor-corrected values are plotted in pg of IL-17F per mL of neat sample (mean +/- SD, n=2).

    • Sandwich ELISA - Mouse IL-17F ELISA Kit (ab204522)
      Sandwich ELISA - Mouse IL-17F ELISA Kit (ab204522)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab204522? Please let us know so that we can cite the reference in this datasheet.

    ab204522 has not yet been referenced specifically in any publications.

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