Mouse IL-17F ELISA Kit (ab204522)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.1 pg/ml
- Range: 9.375 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IL-17F ELISA Kit
See all IL-17F kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 8.4% Inter-assay Sample n Mean SD CV% Overall 3 4.5% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.1 pg/ml -
Range
9.375 pg/ml - 600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 118.6 114.8% - 121.8% Cell culture media 91.9 84.4% - 98.8% Hep Plasma 118.1 107.7% - 124.2% EDTA Plasma 116.6 112.8% - 123% Cit plasma 103.6 96.8% - 113.2% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Goat, Pig -
Product overview
Mouse IL-17F ELISA Kit (ab204522) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-17F protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-17F with 0.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
IL-17F is an IL-17 family member cytokine that stimulates the production of other cytokines such as IL-6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. IL-17F stimulates PBMC and T-cell proliferation. IL-17F inhibits angiogenesis. IL-17F is a glycosylated, disulfide-linked homodimer. IL-17F is also present as IL-17A/F heterodimer. IL-17F is expressed in activated, but not resting, CD4+ T-cells and activated monocytes. It is secreted.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse IL-17F Capture Antibody 1 x 600µl 10X Mouse IL-17F Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Mouse IL-17F Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover (PubMed:11591732, PubMed:11591768, PubMed:11574464). Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis (PubMed:11591732). Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation. -
Tissue specificity
Expressed in activated, but not resting, CD4+ T-cells and activated monocytes. -
Involvement in disease
Candidiasis, familial, 6 -
Sequence similarities
Belongs to the IL-17 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CANDF6
- Cytokine ML 1
- Cytokine ML-1
see all -
Database links
- Entrez Gene: 257630 Mouse
- SwissProt: Q7TNI7 Mouse
- Unigene: 222807 Mouse
Associated products
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Alternative Versions
Images
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Other - Mouse IL-17F ELISA Kit (ab204522)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of IL-17F standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of IL-17F in mouse serum and plasma samples.The concentrations of IL-17F were measured in duplicates, interpolated from the IL-17F standard curve and corrected for sample dilution. Note that 1X Diluted samples were pre-diluted to 25%. The interpolated, dilution factor-corrected values are plotted in pg of IL 17F per mL of neat sample (mean +/- SD, n=2).
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PMA + IL-1a treatment of mouse spleen induces secretion of IL-17F.Mouse spleen cells were cultured in the absence or presence of 10 ng/mL PMA + 10 ng/mL Mouse recombinant IL-1a for 4 days. The cell culture supernatant samples were collected. The concentrations of IL-17F were measured in the diluted supernatants in duplicates, interpolated from the IL-17F standard curve and corrected for sample dilution. Note that 1X Diluted supernatant samples were neat. The interpolated, dilution factor-corrected values are plotted in pg of IL-17F per mL of neat sample (mean +/- SD, n=2).
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Sandwich ELISA - Mouse IL-17F ELISA Kit (ab204522)To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab204522 has been referenced in 1 publication.
- Hu G & Zhou X Gallic Acid Ameliorates Atopic Dermatitis-Like Skin Inflammation Through Immune Regulation in a Mouse Model. Clin Cosmet Investig Dermatol 14:1675-1683 (2021). PubMed: 34815684