Mouse IL-5 ELISA Kit (ab100711)
Key features and details
- Sensitivity: 1 pg/ml
- Range: 2.05 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IL-5 ELISA Kit
See all IL-5 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 1 pg/ml -
Range
2.05 pg/ml - 500 pg/ml -
Recovery
94 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 95.76 85% - 104% Serum 93.44 82% - 102% Plasma 95.49 93% - 104% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s IL-5 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-5 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse IL-5 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get better reproducibility in only 90 minutes with Mouse IL-5 ELISA Kit (ab204523) from our SimpleStep ELISA® range.
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Notes
The standard is affinity purified.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse IL-5 2 vials IL-5 Microplate (12 x 8 wells) 1 unit Recombinant mouse IL-5 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells. -
Sequence similarities
Belongs to the IL-5 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- B-cell differentiation factor I
- Colony stimulating factor
- EDF
see all -
Database links
- Entrez Gene: 16191 Mouse
- SwissProt: P04401 Mouse
- Unigene: 4461 Mouse
Associated products
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SimpleStep ELISA kits
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab100711 has been referenced in 3 publications.
- Zhuang Z et al. Anti-asthmatic miR-224-5p inhibits the FHL1/MAPK pathway to repress airway smooth muscle cell proliferation in a murine model of asthma-like airway inflammation. Allergy Asthma Clin Immunol 18:88 (2022). PubMed: 36184652
- Hu G & Zhou X Gallic Acid Ameliorates Atopic Dermatitis-Like Skin Inflammation Through Immune Regulation in a Mouse Model. Clin Cosmet Investig Dermatol 14:1675-1683 (2021). PubMed: 34815684
- Kong R & Yin J Efficacy of subcutaneous immunotherapy in a mouse model of Humulus pollen-induced allergic asthma. Am J Transl Res 11:280-289 (2019). PubMed: 30787986