Mouse IL-6 R alpha ELISA Kit (ab203360)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 12.6 pg/ml
- Range: 31.3 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse IL-6 R alpha ELISA Kit
See all IL-6R kits
Intra-assay Sample n Mean SD CV% Mouse serum 5 1.8% Inter-assay Sample n Mean SD CV% Mouse serum 3 12%
Sample typeCell culture supernatant, Urine, Serum, Plasma
Assay typeSandwich (quantitative)
Range31.3 pg/ml - 2000 pg/ml
Sample specific recovery Sample type Average % Range Urine 85 80% - 89% Serum 105 103% - 106% Cell culture media 88 84% - 93% Hep Plasma 103 101% - 105% EDTA Plasma 103 102% - 103% Cit plasma 104 100% - 107%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Mouse
Does not react with: Goat, Cow, Pig
Mouse IL-6 R alpha ELISA Kit (ab203360) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-6 R alpha protein in cell culture supernatant, serum, urine, and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-6 R alpha with 12.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Interleukin-6 Receptor (IL-6R, IL-6 Receptor), also known as Interleukin-6 Receptor subunit alpha and membrane glycoprotein 80, is part of a receptor that binds Interleukin-6 (IL-6). IL-6 Receptor weakly binds IL-6 with a low affinity, and then is presented to gp130 to facilitate transition into a high affinity signaling competent hexamer. Activation may lead to regulation of the immune response, acute-phase reactions and hematopoiesis. The production of IL-6 Receptor has been implicated in the pathogenesis of many diseases, including multiple myeloma, auto immune diseases and prostate cancer.IL-6 Receptor has two isoforms. The first is a transmembrane protein in which the N-terminal extracellular portion can be released by proteolytic cleavage. The second isoform is a secreted form that lacks the transmembrane and intracellular portions. This assay is directed at the extracellular portion of IL-6 Receptor and detects both the soluble and membrane bound forms.
PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Mouse IL-6 R alpha Capture Antibody 1 x 600µl 10X Mouse IL-6 R alpha Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Mouse IL-6 R alpha Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
FunctionPart of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity.
Tissue specificityIsoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 3 subfamily.
Contains 1 fibronectin type-III domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
DomainThe two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
modificationsA short soluble form may also be released from the membrane by proteolysis.
Cellular localizationSecreted and Basolateral cell membrane.
- Information by UniProt
- CD 126
- CD126 antigen
- Entrez Gene: 16194 Mouse
- SwissProt: P22272 Mouse
- Unigene: 2856 Mouse
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Standard curve comparison between mouse IL-6 R alpha SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 5-fold increase in sensitivity.
Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of IL-6 R alpha were measured in duplicate and interpolated from the IL-6 R alpha standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 R alpha concentration was determined to be 20.9 ng/mL in mouse serum, 19.1 ng/mL in mouse plasma (Citrate), and 17.1 ng/mL in mouse plasma (EDTA).
The concentrations of IL-6 R alpha were measured in duplicate and interpolated from the IL-6 R alpha standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 R alpha concentration was determined to be 17,650 pg/mL in mouse plasma (heparin), and 849 pg/mL in J774A.1 cell culture supernatant.
J774A.1 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. IL-6 R alpha was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated J774A.1 and cell culture media. Measured values were interpolated from the IL-6 R alpha Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed, 568 pg/mL unstimulated, 849 pg/mL stimulated, and undetectable in media.
To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
ab203360 has been referenced in 4 publications.
- Yousif AS et al. The persistence of interleukin-6 is regulated by a blood buffer system derived from dendritic cells. Immunity 54:235-246.e5 (2021). PubMed: 33357409
- Liu X et al. The protective role of miR-132 targeting HMGA2 through the PI3K/AKT pathway in mice with Alzheimer's disease. Am J Transl Res 13:4632-4643 (2021). PubMed: 34150043
- Serizawa K et al. Anti-IL-6 receptor antibody improves pain symptoms in mice with experimental autoimmune encephalomyelitis. J Neuroimmunol 319:71-79 (2018). ELISA ; Mouse . PubMed: 29685293
- Lee EJ et al. Radiation Inhibits Interleukin-12 Production via Inhibition of C-Rel through the Interleukin-6/ Signal Transducer and Activator of Transcription 3 Signaling Pathway in Dendritic Cells. PLoS One 11:e0146463 (2016). PubMed: 26745884