Mouse Mesothelin ELISA Kit (ab204528)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 14 pg/ml
- Range: 125 pg/ml - 8000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Mesothelin ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 8.3% Inter-assay Sample n Mean SD CV% Overall 3 9.8% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
14 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 119.6 115.6% - 122.8% Cell culture media 88.5 85.6% - 90% EDTA Plasma 116.3 114.4% - 118.8% Cit plasma 112.1 108.2% - 114.6% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Pig -
Product overview
Mouse Mesothelin ELISA Kit (ab204528) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mesothelin protein in cell culture supernatant, serum, tissue extracts, cit plasma, edta plasma, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Mesothelin with 14 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Mesothelin is expressed as a much larger, 70 kDa precursor, which is cleaved into two functional proteins, Megakaryocyte potentiating factor and Mesothelin. Mesothelin is a glycosylated cell-surface protein, anchored to the plasma membrane via its GPI anchor. Membrane-anchored forms of Mesothelin may play a role in cellular adhesion.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Mesothelin Capture Antibody 1 x 600µl 10X Mouse Mesothelin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Mouse Mesothelin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Membrane-anchored forms may play a role in cellular adhesion.
Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. -
Tissue specificity
Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). -
Involvement in disease
Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. -
Sequence similarities
Belongs to the mesothelin family. -
Post-translational
modificationsBoth MPF and the cleaved form of mesothelin are N-glycosylated.
Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. -
Cellular localization
Secreted and Cell membrane. Golgi apparatus. - Information by UniProt
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Alternative names
- CAK1 antigen
- cleaved form
- Megakaryocyte potentiating factor
see all -
Database links
- Entrez Gene: 56047 Mouse
- SwissProt: Q61468 Mouse
- Unigene: 17510 Mouse
Associated products
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Alternative Versions
Images
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Other - Mouse Mesothelin ELISA Kit (ab204528)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Mesothelin standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of Mesothelin in mouse serum and plasma samples.The concentrations of Mesothelin were measured in duplicates, interpolated from the Mesothelin standard curve and corrected for sample dilution. Note that 1X Diluted samples were pre-diluted to 50%. The interpolated, dilution factor-corrected values are plotted in pg of Mesothelin per mL of neat sample (mean +/- SD, n=2).
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Interpolated concentrations of Mesothelin in mouse cell extract samples.The concentrations of Mesothelin were measured in duplicates, interpolated from the Mesothelin standard curve and corrected for sample dilution. Note that 1X Diluted C2C12 cell extract samples were pre-diluted to 125 µg/mL. Note that 1X Diluted NIH/3T3 cell extract samples were pre-diluted to 250 µg/mL. The interpolated, dilution factor-corrected values are plotted in pg of Mesothelin per mg of extract (mean +/- SD, n=2).
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Sandwich ELISA - Mouse Mesothelin ELISA Kit (ab204528)To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab204528 has not yet been referenced specifically in any publications.