Mouse MMP-12 ELISA Kit (ab213878)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse MMP-12 ELISA Kit
See all MMP12 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 337pg/ml 18.2 5.4% 2 16 1472pg/ml 92.74 6.3% 3 16 2522pg/ml 121.1 4.8% Inter-assay Sample n Mean SD CV% 1 24 426pg/ml 27.69 6.5% 2 24 1538pg/ml 109.2 7.1% 3 24 2768pg/ml 157.8 5.7% -
Sample type
Cell culture supernatant, Serum, Hep Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
The Mouse MMP-12 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213878) is designed for the quantitative detection of Mouse MMP-12 in cell culture supernatants, serum and plasma (heparin).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A polycloncal antibody from goat specific for MMP-12 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: A18-C462) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse MMP-12 amount of sample captured in plate.
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Notes
Matrix metalloproteinase-12 (MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may be involved in tissue injury and remodeling.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Mouse MMP-12 coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Mouse MMP-12 antibody 1 x 100µl Lyophilized recombinant Mouse MMP-12 standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
Research areas
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Function
May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. -
Tissue specificity
Found in alveolar macrophages but not in peripheral blood monocytes. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- EC 3.4.24.65
- HME
- Macrophage elastase
see all -
Database links
- Entrez Gene: 17381 Mouse
- SwissProt: P34960 Mouse
- Unigene: 2055 Mouse
Images
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Mouse MMP-12 ELISA Kit (ab213878) Standard Curve
Mouse MMP-12 ELISA Kit (ab213878) Standard Curve
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab213878 has been referenced in 4 publications.
- Spix B et al. Lung emphysema and impaired macrophage elastase clearance in mucolipin 3 deficient mice. Nat Commun 13:318 (2022). PubMed: 35031603
- Mierzejewski B et al. The miR151 and miR5100 Transfected Bone Marrow Stromal Cells Increase Myoblast Fusion in IGFBP2 Dependent Manner. Stem Cell Rev Rep 18:2164-2178 (2022). PubMed: 35190967
- Makino A et al. RSV infection-elicited high MMP-12-producing macrophages exacerbate allergic airway inflammation with neutrophil infiltration. iScience 24:103201 (2021). PubMed: 34703996
- Cottage CT et al. Targeting p16-induced senescence prevents cigarette smoke-induced emphysema by promoting IGF1/Akt1 signaling in mice. Commun Biol 2:307 (2019). PubMed: 31428695