Mouse MMP8 ELISA Kit (ab206982)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 53 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse MMP8 ELISA Kit
See all MMP8 kits
Intra-assay Sample n Mean SD CV% Serum 5 4.9% Inter-assay Sample n Mean SD CV% Serum 3 4.5%
Sample typeCell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma
Assay typeSandwich (quantitative)
Range62.5 pg/ml - 4000 pg/ml
Sample specific recovery Sample type Average % Range Serum 111 108% - 119% Cell culture media 100 98% - 105% Hep Plasma 100 95% - 103% EDTA Plasma 104 97% - 112% Cit plasma 108 105% - 113%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Mouse
Mouse MMP8 ELISA Kit (ab206982) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MMP8 protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MMP8 with 53 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
MMP8 is a member of the matrix metalloproteinase family of zinc proteases that degrade extracellular matrix. MMP8 is expressed in neutrophils and is also known as neutrophil collagenase. MMP8 is expressed as a propeptide. This antibodies in this assay were raised to the mature portion of MMP8.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Mouse MMP8 Capture Antibody 1 x 600µl 10X Mouse MMP8 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Mouse MMP8 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
FunctionCan degrade fibrillar type I, II, and III collagens.
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localizationCytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules.
- Information by UniProt
- CLG 1
- Collagenase 1
- Entrez Gene: 17394 Mouse
- SwissProt: O70138 Mouse
- Unigene: 16415 Mouse
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of MMP8 were measured in duplicate and interpolated from the MMP8 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP8 concentration was determined to be 46.5 ng/mL in mouse serum, 45.2 ng/mL in mouse plasma (Citrate), 36.6 ng/mL in mouse plasma (heparin), and 20.2 ng/mL in mouse plasma (EDTA).
To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
ab206982 has been referenced in 2 publications.
- Tajerian M & Clark JD Spinal matrix metalloproteinase 8 regulates pain after peripheral trauma. J Pain Res 12:1133-1138 (2019). PubMed: 31118746
- Finlay TM et al. Murine neutrophils treated with alphaB-crystallin reduce IL-12p40 production by dendritic cells. Immunology 155:72-84 (2018). PubMed: 29532462