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    products/elisa/mouse-pai1-elisa-kit-serpine1-ab197752.pdf

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RecombinantSimpleStep

Mouse PAI1 ELISA Kit (SERPINE1) (ab197752)

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  • SDS
  • Protocol Booklet
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Other - Mouse PAI1 ELISA Kit (SERPINE1) (ab197752)
  • Example of PAI1 standard curve.
  • Interpolated concentrations of PAI1 in mouse serum and plasma (citrate).
  • Interpolated concentrations of native PAI1 in mouse L929 cell culture supernatant samples treated with PMA and PHA for 3 days.
  • Comparison of secreted PAI1 in unstimulated and PMA/PHA-stimulated L929 Cells.
  • Sandwich ELISA - Mouse PAI1 ELISA Kit (SERPINE1) (ab197752)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 200 pg/ml
  • Range: 298 pg/ml - 5000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

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Overview

  • Product name

    Mouse PAI1 ELISA Kit (SERPINE1)
    See all PAI1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 6.7%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 14.2%
  • Sample type

    Cell culture supernatant, Serum, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    200 pg/ml
  • Range

    298 pg/ml - 5000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 109 90% - 121%
    Cell culture media 83 72% - 88%
    Cit plasma 104 96% - 111%
    serum free media 117 104% - 128%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Mouse PAI1 ELISA Kit (SERPINE1) (ab197752) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PAI1 (SERPINE1) protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PAI1 (SERPINE1) with 200 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Plasminogen activator inhibitor-1 (PAI1 or Serpin E1) is encoded by the Serpine1 gene. PAI1 is a serine protease inhibitor with a major role in the regulation of fibrinolysis. PAI1 is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), both of which are activators of plasminogen. PAI1 is a secreted protein produced by the endothelium as well as adipose tissue.

  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse PAI1 Capture Antibody 1 x 600µl
    10X Mouse PAI1 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Mouse PAI1 Lyophilized Recombinant Protein (ab93068) 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Cardiovascular
    • Blood
    • Fibrinolysis / Thrombolysis
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Protease inhibitors
    • Serine protease inhibitors
    • SERPINs
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Protease inhibitors
    • Other protease inhibitors
    • Cancer
    • Tumor biomarkers
    • Other
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Protease inhibitors ELISA kits
    • Metabolism
    • Types of disease
    • Metabolic disorders
  • Function

    This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
  • Tissue specificity

    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • Involvement in disease

    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • Sequence similarities

    Belongs to the serpin family.
  • Post-translational
    modifications

    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P05121 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Clade E
    • Endothelial plasminogen activator inhibitor
    • Nexin
    • Nexin plasminogen activator inhibitor type 1
    • PAI
    • PAI 1
    • PAI-1
    • PAI1_HUMAN
    • PLANH1
    • Plasminogen activator inhibitor 1
    • Plasminogen activator inhibitor type 1
    • Serine (or cysteine) proteinase inhibitor
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • Serine proteinase inhibitor clade E member 1
    • serpin
    • Serpin E1
    • Serpin peptidase inhibitor clade E
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • Serpine 1
    • SERPINE1
    see all
  • Database links

    • Entrez Gene: 18787 Mouse
    • SwissProt: P22777 Mouse
    • Unigene: 250422 Mouse

    Associated products

    • Alternative Versions

      • Mouse PAI Antibody Pair - BSA and Azide free (ab241696)

    Images

    • Other - Mouse PAI1 ELISA Kit (SERPINE1) (ab197752)
      Other - Mouse PAI1 ELISA Kit (SERPINE1) (ab197752)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of PAI1 standard curve.
      Example of PAI1 standard curve.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of PAI1 in mouse serum and plasma (citrate).
      Interpolated concentrations of PAI1 in mouse serum and plasma (citrate).

      The concentrations of PAI1 were measured in duplicate and interpolated from the PAI1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAI1 concentration was determined to be 18.98 ng/mL in mouse serum and 7.69 ng/mL in mouse plasma (citrate).

    • Interpolated concentrations of native PAI1 in mouse L929 cell culture supernatant samples treated with PMA and PHA for 3 days.
      Interpolated concentrations of native PAI1 in mouse L929 cell culture supernatant samples treated with PMA and PHA for 3 days.
    • Comparison of secreted PAI1 in unstimulated and PMA/PHA-stimulated L929 Cells.
      Comparison of secreted PAI1 in unstimulated and PMA/PHA-stimulated L929 Cells.

      L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. PAI1 was measured in 10-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the PAI1 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 17.9 ng/mL unstimulated, 31.1 ng/mL stimulated, and undetectable in media.

    • Sandwich ELISA - Mouse PAI1 ELISA Kit (SERPINE1) (ab197752)
      Sandwich ELISA - Mouse PAI1 ELISA Kit (SERPINE1) (ab197752)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (5)

    Publishing research using ab197752? Please let us know so that we can cite the reference in this datasheet.

    ab197752 has been referenced in 5 publications.

    • Nichols CE  et al. Lrp1 Regulation of Pulmonary Function. Follow-Up of Human GWAS in Mice. Am J Respir Cell Mol Biol 64:368-378 (2021). PubMed: 33290178
    • Ghosh AK  et al. Pharmacological inhibition of PAI-1 alleviates cardiopulmonary pathologies induced by exposure to air pollutants PM2.5. Environ Pollut 287:117283 (2021). PubMed: 34426376
    • Wiley CD  et al. SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells. Cell Rep 28:3329-3337.e5 (2019). PubMed: 31553904
    • Li B  et al. MicroRNA-30b protects myocardial cell function in patients with acute myocardial ischemia by targeting plasminogen activator inhibitor-1. Exp Ther Med 15:5125-5132 (2018). PubMed: 29805539
    • Ma J  et al. Rivaroxaban attenuates thrombosis by targeting the NF-?B signaling pathway in a rat model of deep venous thrombus. Int J Mol Med 40:1869-1880 (2017). PubMed: 29039441

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