Mouse PAI1 ELISA Kit (SERPINE1) (ab197752)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 200 pg/ml
- Range: 298 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse PAI1 ELISA Kit (SERPINE1)
See all PAI1 kits
Intra-assay Sample n Mean SD CV% Serum 5 6.7% Inter-assay Sample n Mean SD CV% Serum 3 14.2%
Sample typeCell culture supernatant, Serum, Cit plasma
Assay typeSandwich (quantitative)
Range298 pg/ml - 5000 pg/ml
Sample specific recovery Sample type Average % Range Serum 109 90% - 121% Cell culture media 83 72% - 88% Cit plasma 104 96% - 111% serum free media 117 104% - 128%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Mouse
Mouse PAI1 ELISA Kit (SERPINE1) (ab197752) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PAI1 (SERPINE1) protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PAI1 (SERPINE1) with 200 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Plasminogen activator inhibitor-1 (PAI1 or Serpin E1) is encoded by the Serpine1 gene. PAI1 is a serine protease inhibitor with a major role in the regulation of fibrinolysis. PAI1 is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), both of which are activators of plasminogen. PAI1 is a secreted protein produced by the endothelium as well as adipose tissue.
PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Mouse PAI1 Capture Antibody 1 x 600µl 10X Mouse PAI1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Mouse PAI1 Lyophilized Recombinant Protein (ab93068) 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
FunctionThis inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
Tissue specificityFound in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
Involvement in diseaseDefects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
Sequence similaritiesBelongs to the serpin family.
modificationsInactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
- Information by UniProt
- Clade E
- Endothelial plasminogen activator inhibitor
- Entrez Gene: 18787 Mouse
- SwissProt: P22777 Mouse
- Unigene: 250422 Mouse
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of PAI1 were measured in duplicate and interpolated from the PAI1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAI1 concentration was determined to be 18.98 ng/mL in mouse serum and 7.69 ng/mL in mouse plasma (citrate).
L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. PAI1 was measured in 10-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the PAI1 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 17.9 ng/mL unstimulated, 31.1 ng/mL stimulated, and undetectable in media.
To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
ab197752 has been referenced in 5 publications.
- Nichols CE et al. Lrp1 Regulation of Pulmonary Function. Follow-Up of Human GWAS in Mice. Am J Respir Cell Mol Biol 64:368-378 (2021). PubMed: 33290178
- Ghosh AK et al. Pharmacological inhibition of PAI-1 alleviates cardiopulmonary pathologies induced by exposure to air pollutants PM2.5. Environ Pollut 287:117283 (2021). PubMed: 34426376
- Wiley CD et al. SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells. Cell Rep 28:3329-3337.e5 (2019). PubMed: 31553904
- Li B et al. MicroRNA-30b protects myocardial cell function in patients with acute myocardial ischemia by targeting plasminogen activator inhibitor-1. Exp Ther Med 15:5125-5132 (2018). PubMed: 29805539
- Ma J et al. Rivaroxaban attenuates thrombosis by targeting the NF-?B signaling pathway in a rat model of deep venous thrombus. Int J Mol Med 40:1869-1880 (2017). PubMed: 29039441