Mouse TGF beta 1 ELISA Kit (ab119557)
Key features and details
- Sensitivity: 7.8 pg/ml
- Range: 31.3 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse TGF beta 1 ELISA Kit
See all TGF beta 1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4 7.9% Inter-assay Sample n Mean SD CV% Overall 4 5.8% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
7.8 pg/ml -
Range
31.3 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 124% - 141% Serum 92% - 103% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s TGF beta 1 Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Mouse TGF beta 1 concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate).
Mouse TGF beta 1 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Mouse TGF beta 1 detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after the addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Mouse TGF beta 1 captured on the plate
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Assay Buffer Concentrate 2 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 6 units Biotinylated mouse anti-TGF beta 1 monoclonal antibody 1 x 120µl Microplate coated with monoclonal antibody to mouse TGF beta 1 (12 x 8 wells) 1 unit Mouse TGF beta 1 Standard lyophilized 2 vials Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml -
Research areas
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Function
Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. -
Tissue specificity
Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. -
Involvement in disease
Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision. -
Sequence similarities
Belongs to the TGF-beta family. -
Post-translational
modificationsGlycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- Cartilage-inducing factor
- CED
- Differentiation inhibiting factor
see all -
Database links
- Entrez Gene: 21803 Mouse
- SwissProt: P04202 Mouse
- Unigene: 248380 Mouse
Images
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Sandwich ELISA - TGF beta 1 Mouse ELISA Kit (ab119557)Standard curve of Ms TGFb, with background signal subtracted (duplicates; +/- SD).
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Sandwich ELISA - TGF beta 1 Mouse ELISA Kit (ab119557)Mouse TGFb measured in activated biological fluids, background signal subtracted (duplicates +/- SD). * Samples without activation. -
Typical Standard CurveRepresentative Standard Curve using ab119557.
Datasheets and documents
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SDS download
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Datasheet download
References (33)
ab119557 has been referenced in 33 publications.
- Nishad R et al. Growth hormone induces mitotic catastrophe of glomerular podocytes and contributes to proteinuria. Cell Death Dis 12:342 (2021). PubMed: 33795655
- He Y et al. Receptor Tyrosine Kinase Inhibitor Sunitinib as Novel Immunotherapy to Inhibit Myeloid-Derived Suppressor Cells for Treatment of Endometriosis. Front Immunol 12:641206 (2021). PubMed: 34367125
- Permyakova A et al. A Novel Indoline Derivative Ameliorates Diabesity-Induced Chronic Kidney Disease by Reducing Metabolic Abnormalities. Front Endocrinol (Lausanne) 11:91 (2020). PubMed: 32218769
- Jia J et al. Cutaneous neurofibroma cells with active YAP promotes proliferation of macrophages resulting in increased accumulation of macrophages by modulating CCL5 and TGF-ß1. Oncol Rep 43:1319-1330 (2020). PubMed: 32323813
- Li J et al. HOXC10 promotes cell migration, invasion, and tumor growth in gastric carcinoma cells through upregulating proinflammatory cytokines. J Cell Physiol 235:3579-3591 (2020). PubMed: 31552684