Mouse VCAM1 ELISA Kit (CD106) (ab201278)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 5.59 pg/ml
- Range: 46.9 pg/ml - 3000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
-
Product name
Mouse VCAM1 ELISA Kit (CD106)
See all VCAM1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 8 4.3% Inter-assay Sample n Mean SD CV% Overall 3 5.6% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
5.59 pg/ml -
Range
46.9 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 107 104% - 109% Cell culture media 87 86% - 88% Hep Plasma 108 107% - 108% EDTA Plasma 109 106% - 111% Cit plasma 111 109% - 113% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Goat, Cow, Pig -
Product overview
Mouse VCAM1 ELISA Kit (CD106) (ab201278) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VCAM1 (CD106) protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse VCAM1 (CD106) with 5.59 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
VCAM1 is important in cell-cell recognition, and appears to function in leukocyte-endothelial cell adhesion. It interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse VCAM1 (CD106) Capture Antibody 1 x 600µl 10X Mouse VCAM1 (CD106) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CP 1 x 6ml Mouse VCAM1 (CD106) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. -
Tissue specificity
Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue. -
Sequence similarities
Contains 7 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion. -
Post-translational
modificationsSialoglycoprotein. -
Cellular localization
Membrane. - Information by UniProt
-
Alternative names
- CD106
- CD106 Antigen
- INCAM 100
see all -
Database links
- Entrez Gene: 22329 Mouse
- SwissProt: P29533 Mouse
- Unigene: 440909 Mouse
- Unigene: 76649 Mouse
Associated products
-
Alternative Versions
Images
-
Other - Mouse VCAM1 ELISA Kit (CD106) (ab201278)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Mouse VCAM1 (CD106) standard curve comparison data.Standard curve comparison between mouse VCAM1 (CD106) SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 9-fold increase in sensitivity.
-
Example of VCAM1 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
-
Example of VCAM1 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
-
Titration of mouse serum, citrate plasma, EDTA plasma, and Heparin plasma within the working range of the assay.The 1X dilution was 0.2% for each biological. Interpolated data values (mean +/- SD, n = 2) are graphed.
-
Titration of mouse unstimulated (-) Lung and L929 extracts within the working range of the assay.The 1X dilution was an extract load of 250 µg/mL. Interpolated data values (mean +/- SD, n = 2) are graphed.
-
Sandwich ELISA - Mouse VCAM1 ELISA Kit (CD106) (ab201278)To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab201278 has not yet been referenced specifically in any publications.