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    products/elisa/mouseinterferon-alpha-1-elisa-kit-ab252352.pdf

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Immunology Innate Immunity Cytokines Interferons
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RecombinantSimpleStep

Mouse Interferon alpha 1 ELISA Kit (ab252352)

  • Datasheet
  • SDS
  • Protocol Booklet
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Example of mouse IFN-alpha standard curve in Sample Diluent NS.
  • Other - Mouse IFN-alpha ELISA Kit (ab252352)
  • Interpolated concentrations of spike IFN-alpha in mouse serum, plasma and cell culture media samples.
  • Sandwich ELISA - Mouse IFN-alpha ELISA Kit (ab252352)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 7 pg/ml
  • Range: 15.6 pg/ml - 1000 pg/ml
  • Sample type: Cell culture media, Cit plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

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Overview

  • Product name

    Mouse Interferon alpha 1 ELISA Kit
    See all Interferon alpha 1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    serum 8 2.6%
    Inter-assay
    Sample n Mean SD CV%
    serum 3 7.7%
  • Sample type

    Serum, Cell culture media, Hep Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    7 pg/ml
  • Range

    15.6 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 89 82% - 95%
    Cell culture media 100 95% - 106%
    Hep Plasma 89 88% - 91%
    Cit plasma 89 85% - 92%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Mouse Interferon alpha ELISA Kit (ab252352) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IFN-alpha protein in cell culture media, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IFN-alpha with 7 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse Interferon alpha 1 Capture Antibody 1 x 600µl
    10X Mouse Interferon alpha 1 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BR 1 x 6ml
    Mouse Interferon alpha 1 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interferons
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interferons
    • Neuroscience
    • Processes
  • Function

    Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
  • Sequence similarities

    Belongs to the alpha/beta interferon family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P01562 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • IFL
    • IFN
    • IFN alpha
    • IFN alpha 1/13
    • IFN alpha 1b
    • IFN alphaD
    • IFN-alpha-1/13
    • IFNA@
    • IFNA1
    • IFNA1_HUMAN
    • IFNA13
    • Interferon alpha 1
    • Interferon alpha-1/13
    • Interferon alpha-D
    • Interferon alpha1
    • Interferon, alpha 13
    • LeIF D
    see all
  • Database links

    • Entrez Gene: 15962 Mouse
    • Entrez Gene: 230396 Mouse
    • SwissProt: P01572 Mouse

    Associated products

    • Alternative Versions

      • Mouse Interferon alpha 1 Antibody Pair - BSA and Azide free (ab256697)

    Images

    • Example of mouse IFN-alpha standard curve in Sample Diluent NS.
      Example of mouse IFN-alpha standard curve in Sample Diluent NS.

      The IFN-alpha standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Other - Mouse IFN-alpha ELISA Kit (ab252352)
      Other - Mouse IFN-alpha ELISA Kit (ab252352)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Interpolated concentrations of spike IFN-alpha in mouse serum, plasma and cell culture media samples.
      Interpolated concentrations of spike IFN-alpha in mouse serum, plasma and cell culture media samples.

      The concentrations of IFN-alpha were measured in duplicates, interpolated from the IFN-alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, and RPMI cell culture media containing 10% fetal bovine serum 100%.

    • Sandwich ELISA - Mouse IFN-alpha ELISA Kit (ab252352)
      Sandwich ELISA - Mouse IFN-alpha ELISA Kit (ab252352)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab252352? Please let us know so that we can cite the reference in this datasheet.

    ab252352 has been referenced in 3 publications.

    • Lai Y  et al. Ganghuo Kanggan Decoction in Influenza: Integrating Network Pharmacology and In Vivo Pharmacological Evaluation. Front Pharmacol 11:607027 (2020). PubMed: 33362562
    • Lin C  et al. Intratumoral Delivery of a PD-1-Blocking scFv Encoded in Oncolytic HSV-1 Promotes Antitumor Immunity and Synergizes with TIGIT Blockade. Cancer Immunol Res 8:632-647 (2020). PubMed: 32127389
    • Xu H  et al. Inhibition of TLR4 inhibits allergic responses in murine allergic rhinitis by regulating the NF-?B pathway. Exp Ther Med 18:761-768 (2019). PubMed: 31281453

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