Mouse Interferon alpha 1 ELISA Kit (ab252352)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 7 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture media, Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Interferon alpha 1 ELISA Kit
See all Interferon alpha 1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 8 2.6% Inter-assay Sample n Mean SD CV% serum 3 7.7% -
Sample type
Serum, Cell culture media, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
7 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 89 82% - 95% Cell culture media 100 95% - 106% Hep Plasma 89 88% - 91% Cit plasma 89 85% - 92% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Interferon alpha ELISA Kit (ab252352) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IFN-alpha protein in cell culture media, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IFN-alpha with 7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10 x 96 tests 10X Mouse Interferon alpha 1 Capture Antibody 1 x 600µl 1 x 6000µl 10X Mouse Interferon alpha 1 Detector Antibody 1 x 600µl 1 x 6000µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 200ml Antibody Diluent 4BR 1 x 6ml 10 x 6ml Mouse Interferon alpha 1 Lyophilized Recombinant Protein 2 vials 2 x 10 vials Plate Seals 1 unit 1 x 10 units Sample Diluent NS (ab193972) 1 x 50ml 2 x 250ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 x 10 units Stop Solution 1 x 12ml 1 x 120ml TMB Development Solution 1 x 12ml 1 x 120ml -
Research areas
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Function
Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. -
Sequence similarities
Belongs to the alpha/beta interferon family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IFL
- IFN
- IFN alpha
see all -
Database links
- Entrez Gene: 15962 Mouse
- Entrez Gene: 230396 Mouse
- SwissProt: P01572 Mouse
Associated products
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Alternative Versions
Images
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Example of mouse IFN-alpha standard curve in Sample Diluent NS.
The IFN-alpha standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Other - Mouse IFN-alpha ELISA Kit (ab252352)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Interpolated concentrations of spike IFN-alpha in mouse serum, plasma and cell culture media samples.The concentrations of IFN-alpha were measured in duplicates, interpolated from the IFN-alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, and RPMI cell culture media containing 10% fetal bovine serum 100%.
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Sandwich ELISA - Mouse IFN-alpha ELISA Kit (ab252352)To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab252352 has been referenced in 6 publications.
- Acevedo Ospina H et al. Macrophage Mitochondrial Biogenesis and Metabolic Reprogramming Induced by Leishmania donovani Require Lipophosphoglycan and Type I Interferon Signaling. mBio 13:e0257822 (2022). PubMed: 36222510
- Yan M et al. Interleukin-7 aggravates myocardial ischaemia/reperfusion injury by regulating macrophage infiltration and polarization. J Cell Mol Med 25:9939-9952 (2021). PubMed: 34581005
- Das A et al. Mutation in Irf8 Gene (Irf8R294C ) Impairs Type I IFN-Mediated Antiviral Immune Response by Murine pDCs. Front Immunol 12:758190 (2021). PubMed: 34867997
- Lai Y et al. Ganghuo Kanggan Decoction in Influenza: Integrating Network Pharmacology and In Vivo Pharmacological Evaluation. Front Pharmacol 11:607027 (2020). PubMed: 33362562
- Lin C et al. Intratumoral Delivery of a PD-1-Blocking scFv Encoded in Oncolytic HSV-1 Promotes Antitumor Immunity and Synergizes with TIGIT Blockade. Cancer Immunol Res 8:632-647 (2020). PubMed: 32127389
- Xu H et al. Inhibition of TLR4 inhibits allergic responses in murine allergic rhinitis by regulating the NF-?B pathway. Exp Ther Med 18:761-768 (2019). PubMed: 31281453