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RecombinantSimpleStep

Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (ab263880)

Submit a review Submit a question References (2)
SimpleStep ELISA Protocol Diagram
  • Example of mouse and rat ACTH standard curve in 1X Cell Extraction Buffer PTR.
  • Interpolated concentrations of native ACTH in mouse and rat serum and plasma samples.
  • Interpolated concentrations of native ACTH in rat serum and plasma samples.
  • Sandwich ELISA - Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (ab263880)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 6 pg/ml
  • Range: 7.81 pg/ml - 500 pg/ml
  • Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone)
    See all ACTH kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Plasma 8 4.2%
    Inter-assay
    Sample n Mean SD CV%
    Plasma 3 6.6%

  • Sample type

    Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    6 pg/ml

  • Range

    7.81 pg/ml - 500 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 87 82% - 92%
    Hep Plasma 116 113% - 120%
    EDTA Plasma 105 100% - 110%
    Cit plasma 101 89% - 107%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse, Rat

  • Product overview

    Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (ab263880) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ACTH (adrenocorticotropic hormone) protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse/Rat ACTH (adrenocorticotropic hormone) with 6 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    ACTH, adrenocorticotropic hormone, is a peptide hormone cleavage product of the POMC protein produced by the pituitary gland. ACTH plays an important role in stress and circadian rhythm signaling. ACTH’s primary action is on the adrenal cortex, where its binding induces the secretion of glucocorticoid steroid hormones. The peptide sequence for this hormone is highly evolutionarily conserved amongst mammals. Specifically, for mouse and rat, this sequence shares 100% identity.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Example of mouse and rat ACTH standard curve in 1X Cell Extraction Buffer PTR.
    Example of mouse and rat ACTH standard curve in 1X Cell Extraction Buffer PTR.

    The ACTH standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native ACTH in mouse and rat serum and plasma samples.
    Interpolated concentrations of native ACTH in mouse and rat serum and plasma samples.

    The concentrations of ACTH were measured in duplicates, interpolated from the ACTH standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50% and plasma (edta) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ACTH concentration was determined to be 123.02 pg/mL in serum, 433.40 pg/mL in plasma (citrate), 240.48 pg/mL in plasma (heparin) and 328.43 pg/mL in plasma (edta).

  • Interpolated concentrations of native ACTH in rat serum and plasma samples.
    Interpolated concentrations of native ACTH in rat serum and plasma samples.

    The concentrations of ACTH were measured in duplicates, interpolated from the ACTH standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 15%, plasma (heparin) 15% and plasma (edta) 15%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ACTH concentration was determined to be 1178.40 pg/mL in serum, 2974.54 pg/mL in plasma (citrate), 1988.24 pg/mL in plasma (heparin) and 1508.42 pg/mL in plasma (edta).

  • Sandwich ELISA - Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (ab263880)
    Sandwich ELISA - Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (ab263880)
    To learn more about the advantages of recombinant antibodies see here.

References (2)

Publishing research using ab263880? Please let us know so that we can cite the reference in this datasheet.

ab263880 has been referenced in 2 publications.

  • Bashiri H  et al. The combination of fluoxetine and environmental enrichment reduces postpartum stress-related behaviors through the oxytocinergic system and HPA axis in mice. Sci Rep 11:8518 (2021). PubMed: 33875712
  • Xu EY  et al. Genetic analysis of the cooperative tumorigenic effects of targeted deletions of tumor suppressors Rb1, Trp53, Men1, and Pten in neuroendocrine tumors in mice. Oncotarget 11:2718-2739 (2020). PubMed: 32733644

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