Nitrotyrosine ELISA Kit (ab210603)
Key features and details
- Sensitivity: 50 nM
- Range: 62.5 nM - 8000 nM
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
Product nameNitrotyrosine ELISA Kit
Intra-assay Sample n Mean SD CV% 3 Samples 30 < 10% Inter-assay Sample n Mean SD CV% 3 Samples 30 < 15%
Sample typeUrine, Serum, Plasma, Other biological fluids, Cell Lysate
Sensitivity= 50 nM
Range62.5 nM - 8000 nM
Assay time2h 00m
Assay durationMultiple steps standard assay
Abcam’s Nitrotyrosine in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab210603) is designed for the competitive measurement of Free Nitrotyrosine protein in plasma, serum, cell lysates, urine, and other sample matrices. The ELISA utilizes an Nitrotyrosine-coated plate and an HRP-conjugated antibody for detection which allows for an assay range of 62.5 to 8000 nM Free Nitrotyrosine, with a sensitivity of 50 nM. The other highlights of this kit are a quick incubation time of 60 minutes, stable reagents, and an easy to use protocol.
Nitrotyrosine has been identified as a marker of inflammation and NO production. Nitrotyrosine is formed in presence of the active metabolite NO. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production. Since nitrotyrosine is a stable end product of peroxynitrite oxidation, assessment of its plasma concentration may be useful as a marker of NO-dependent damage in vivo. Since NOX is only an indicator for enhanced NO production, protein associated nitrotyrosine might be a more suitable marker for damage induced by reactive nitrogen intermediates derived from NO. Furthermore, most proteins have a longer half-life in the circulation than NOX levels. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, celiac disease, rheumatoid arthritis, chronic renal failure and septic shock. In normal plasma low, undetectable, levels of nitrotyrosine are present.
Nitrosylation of the amino acid tyrosine occurs both for free tyrosine and for protein bound tyrosine.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests Anti-Nitrotyrosine:HRP Conjugated Detection Antibody 1 x 75µl Nitrosylated BSA Coated Plate 1 x 96 tests Nitrotyrosine Antibody Diluent Blue 1 x 13ml Nitrotyrosine Standard 1 x 110µl Plate Cover 2 units Sample and Standard Diluent Red 1 x 50ml Stop Solution 1 x 13ml TMB Substrate 1 x 13ml Wash Buffer Concentrate (10X) 1 x 50ml
RelevanceProtein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A, SP-A, to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis.
Datasheets and documents
ab210603 has been referenced in 9 publications.
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- Francesca F et al. Antroquinonol administration in animal preclinical studies for Alzheimer's disease (AD): A new avenue for modifying progression of AD pathophysiology. Brain Behav Immun Health 21:100435 (2022). PubMed: 35252893
- Quinn KM et al. Blood oxidative stress biomarkers in women: influence of oral contraception, exercise, and N-acetylcysteine. Eur J Appl Physiol 122:1949-1964 (2022). PubMed: 35674828
- Morgan HL et al. Paternal low protein diet perturbs inter-generational metabolic homeostasis in a tissue-specific manner in mice. Commun Biol 5:929 (2022). PubMed: 36075960
- Chikopela T et al. Endothelial dysfunction and body mass index: is there a role for plasma peroxynitrite? Beni Suef Univ J Basic Appl Sci 10:N/A (2021). PubMed: 33569505
- Vu LD et al. Deficiency in ST2 signaling ameliorates RSV-associated pulmonary hypertension. Am J Physiol Heart Circ Physiol 321:H309-H317 (2021). PubMed: 34170196
- Chikopela T et al. Arterial stiffness is associated with oxidative stress and endothelial activation among persons with treated HIV in Zambia. South Afr J HIV Med 22:1298 (2021). PubMed: 34858654
- Siervo M et al. Nitrate-Rich Beetroot Juice Reduces Blood Pressure in Tanzanian Adults with Elevated Blood Pressure: A Double-Blind Randomized Controlled Feasibility Trial. J Nutr 150:2460-2468 (2020). PubMed: 32729923
- Ashor AW et al. Effects of inorganic nitrate and vitamin C co-supplementation on blood pressure and vascular function in younger and older healthy adults: A randomised double-blind crossover trial. Clin Nutr N/A:N/A (2019). PubMed: 30930132