Overall Exosome Capture and Quantification ELISA Assay Kit (ab285284)
Key features and details
- Range: 0.78125 µg - 50 µg
- Sample type: Plasma
- Assay type: Sandwich
Overview
-
Product name
Overall Exosome Capture and Quantification ELISA Assay Kit
See all Exosome kits -
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 13% -
Sample type
Plasma -
Assay type
Sandwich -
Range
0.781 µg - 50 µg -
Assay duration
Multiple steps standard assay -
Product overview
Overall Exosome Capture and Quantification ELISA Assay Kit ab285284 (previously called ExoQuant Overall Exosome Capture and Quantification ELISA Assay Kit (Human Plasma, Colorimetric), K1201) is a patented double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes.
It is a successful platform for exosomes quantification and characterization from small amount of human biological fluids (plasma, serum, urine, saliva) or cell media. It may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. The kit consists of ELISA plates pre-coated with proprietary pan-exosome antibodies enabling specific capture of exosomes from different biological samples, including cell culture supernatants and human biological fluids. Quantification and characterization of exosomal proteins is subsequently performed using appropriate detection antibodies against exosome associated antigens that can be for either generic or cell/tissue-specific exosomes. The kit contains Kit contains Lyophilized Exosome Standards for assay calibration. Lyophilized Exosome Standards, characterized for protein content and particle number allow the quantification of unknown sample by a standard calibration curve. Transparent and white plates are available depending on the downstream detection approach (colorimetric and luminometric respectively).
-
Platform
Microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests 25X Wash buffer 2 x 15ml Exosome Standards 2 vials HRP-Conjugate 1 vial Immunoplate (Transparent) 1 unit Primary antibody 1 x 20µl Sample buffer 2 x 10ml Stop Solution 1 x 10ml Substrate chomogenic solutions 1 x 10ml -
Relevance
Exosomes are best defined as extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB), with the plasma membrane. This liberates intraluminal vesicles (ILVs) into the extracellular milieu and the vesicles thereby released are what we know as exosomes. They contain abundant proteins, DNA, mRNA, and non-coding RNAs. They are now considered as an additional mechanism for intercellular communication, allowing cells to exchange proteins, lipids and genetic material. Increasing studies have shown that exosomes play an important role in tumour initiation, growth, progression, metastasis, drug resistance and immune escape.
Images
-
CD9 Titration of COLO1 (1) and plasma (2) exosomes. Example of standard curves obtained. Exosome standard preparation derived from (1) COLO1 Cell Culture Supernatant and (2) Plasma of healthy donors. This standard curve is for demonstration only. For quantification purposes, a standard curve must be obtained with every assay.
-
CD9 marker detection by Western Blotting on lyophilized exosomes from human plasma and the recombinant CD9 protein.
-
CD9 titration of plasma healthy donor exosome standards.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab285284 has not yet been referenced specifically in any publications.