Overall Exosome Capture and Quantification ELISA Assay Kit (ab285284)

Overall Exosome Capture and Quantification ELISA Assay Kit ab285284 (previously called ExoQuant Overall Exosome Capture and Quantification ELISA Assay Kit (Human Plasma, Colorimetric), K1201) is a patented double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes.

It is a successful platform for exosomes quantification and characterization from small amount of human biological fluids (plasma, serum, urine, saliva) or cell media. It may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. The kit consists of ELISA plates pre-coated with proprietary pan-exosome antibodies enabling specific capture of exosomes from different biological samples, including cell culture supernatants and human biological fluids. Quantification and characterization of exosomal proteins is subsequently performed using appropriate detection antibodies against exosome associated antigens that can be for either generic or cell/tissue-specific exosomes. The kit contains Kit contains Lyophilized Exosome Standards for assay calibration. Lyophilized Exosome Standards, characterized for protein content and particle number allow the quantification of unknown sample by a standard calibration curve. Transparent and white plates are available depending on the downstream detection approach (colorimetric and luminometric respectively).