Key features and details
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Semi-quantitative
- Reacts with: Mouse
Product namePhospho-FRS2 (Y436) ELISA Kit
See all FRS2 kits
Sample typeCell Lysate
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Phospho-FRS2 (Y436) ELISA Kit (ab279805) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in mouse cell lysates. By determining phosphorylated FRS2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of mouse phospho-FRS2. An anti-pan FRS2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and FRS2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-FRS2 (Y436) antibody is used to detect phosphorylated FRS2. After washing away unbound antibody, HRP conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FRS2 (Y436) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 2X Cell lysate buffer 1 x 5ml 5X Assay Diluent 1 x 15ml HRP-conjugated anti-rabbit IgG concentrate (500X) 1 vial Pan-FRS2 Coated Microplate 1 unit Positive Control - treated NIH/3T3 cell lysate 1 vial Rabbit anti-phospho-FRS2 (Y436)-antibody 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionAdapter protein that links FGR and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Modulates signaling via SHC1 by competing for a common binding site on NTRK1.
Tissue specificityHighly expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.
Sequence similaritiesContains 1 IRS-type PTB domain.
modificationsPhosphorylated on tyrosine residues upon stimulation by NGF.
Ubiquitinated when tyrosine phosphorylated and in a complex with GRB2. The unphosphorylated form is not subject to ubiquitination.
Cellular localizationEndomembrane system. Cytoplasmic, membrane-bound.
- Information by UniProt
- FGFR signaling adaptor SNT
- FGFR signalling adaptor
- FGFR substrate 2
ab279805 has not yet been referenced specifically in any publications.