For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/elisa/phospho-s232-pdh-e1-alpha-protein-pdha1-profiling-elisa-kit-ab115343.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Metabolism Energy Metabolism
Share by email

Phospho S232 PDH E1 alpha protein (PDHA1) Profiling ELISA Kit (ab115343)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (1)References (3)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) ELISA Kit (ab115343)
  • ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) ELISA Kit (ab115343)
  • ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) ELISA Kit (ab115343)
  • ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) ELISA Kit (ab115343)

Key features and details

  • Sensitivity: 31 µg/ml
  • Range: 31 µg/ml - 1000 µg/ml
  • Sample type: Cell culture extracts, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse, Rat, Cow, Human

You may also be interested in

Kit
Product image
m6A RNA Methylation Assay Kit (Colorimetric) (ab185912)
Primary
Product image
Anti-METTL3 antibody [EPR18810] (ab195352)
Primary
Product image
Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (ab208577)

View more associated products

Overview

  • Product name

    Phospho S232 PDH E1 alpha protein (PDHA1) Profiling ELISA Kit
    See all PDHA1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 8 = 8.3%
    Inter-assay
    Sample n Mean SD CV%
    1 4 = 7.7%
  • Sample type

    Cell culture extracts, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 31 µg/ml
  • Range

    31 µg/ml - 1000 µg/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human
  • Product overview

    ab115343 is an in vitro enzyme-linked immunosorbent assay to determine the levels of phospho S232 PDHA1 protein in cell and tissue lysates. The assay employs a mouse antibody specific for PDHA1 protein coated on a 96-well plate. Samples are pipetted into the wells and PDHA1 protein present in the sample is bound to the wells by the immobilized antibody. The wells are washed and a rabbit anti-phospho S232 PDHA1 protein detector antibody is added. After washing away unbound detector antibody, HRP-conjugated anti-rabbit antibody is pipetted into the wells. The wells are again washed, an HRP substrate solution (TMB) is added to the wells and color develops in proportion to the amount of phospho S232 PDHA1 protein bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.

  • Notes

    Store all components at 4°C. The kits are stable for at least 6

    months. Unused microplate strips should be returned to the pouch

    containing the desiccant and resealed.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Blocking Buffer 1 x 6ml
    10X HRP Label 1 x 1ml
    10X phospho S232 PDHA1 protein Detector Antibody 1 x 0.7ml
    20X Buffer 1 x 20ml
    Extraction Buffer (ab260490) 1 x 15ml
    Microplate 96 antibody coated wells in 12 strips 1 unit
    HRP Development Solution 1 x 12ml
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
  • Function

    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
  • Tissue specificity

    Ubiquitous.
  • Involvement in disease

    Defects in PDHA1 are a cause of pyruvate decarboxylase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (X-LS).
    Defects in PDHA1 are the cause of X-linked Leigh syndrome (X-LS) [MIM:308930]. X-LS is an early-onset progressive neurodegenerative disorder with a characteristic neuropathology consisting of focal, bilateral lesions in one or more areas of the central nervous system, including the brainstem, thalamus, basal ganglia, cerebellum, and spinal cord. The lesions are areas of demyelination, gliosis, necrosis, spongiosis, or capillary proliferation. Clinical symptoms depend on which areas of the central nervous system are involved. The most common underlying cause is a defect in oxidative phosphorylation. LS may be a feature of a deficiency of any of the mitochondrial respiratory chain complexes.
  • Cellular localization

    Mitochondrion matrix.
  • Target information above from: UniProt accession P08559 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • ODPA_HUMAN
    • PDH
    • PDHA
    • PDHA1
    • PDHCE1A
    • PDHE1 A type I
    • PDHE1-A type I
    • PHE1A
    • Pyruvate Dehydrogenase (lipoamide) alpha 1
    • Pyruvate dehydrogenase complex, E1 alpha polypeptide 1
    • Pyruvate Dehydrogenase E1 alpha
    • Pyruvate dehydrogenase E1 component subunit alpha
    • Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
    • Pyruvate Dehydrogenase E1-alpha subunit
    see all
  • Database links

    • Entrez Gene: 407109 Cow
    • Entrez Gene: 5160 Human
    • Entrez Gene: 18597 Mouse
    • Entrez Gene: 29554 Rat
    • Omim: 300502 Human
    • SwissProt: A7MB35 Cow
    • SwissProt: P08559 Human
    • SwissProt: P35486 Mouse
    • SwissProt: P26284 Rat
    • Unigene: 530331 Human
    • Unigene: 34775 Mouse
    • Unigene: 3655 Rat
    see all

Associated products

  • Related Products

    • Phospho S300 PDH E1 alpha protein (PDHA1) Profiling ELISA Kit (ab115345)
  • SimpleStep ELISA kits

    • Human PDH E1 alpha protein ELISA Kit (PDHA1) (ab181415)

Images

  • ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) ELISA Kit (ab115343)
    ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) ELISA Kit (ab115343)

    The PDHA1 bound from undosed HeLa cells was subject to in-well kinase treatment (PDK1&3) or in-well phosphatase treatment (PDP1) according to the supplementary protocol shown below. Untreated cells showed a significant endogenous phosphorylation signal at S232 which is only slightly increased by kinase treatment. Conversely phosphatase treatment was able to significantly reduce the phospho S232 signal from the endogenous levels.

  • ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) ELISA Kit (ab115343)
    ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) ELISA Kit (ab115343)
    HeLa cells were cultured for 4 hours in media supplemented with DCA (20mM) to specifically inhibit mitochondrial PDH kinases, or NaF (20mM), a general inhibitor of serine/threonine protein phosphatases. The DCA treatment reduced the level of phospho S232. Conversely NaF treatment, to inhibit cellular serine phosphatases, increased the phosphorylation level of S232.
  • ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) ELISA Kit (ab115343)
    ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) ELISA Kit (ab115343)

    Example control sample curve for HepG2 cell extract. The phosphorylation state of PDHA1 can vary by treatment but also by cell culture conditions such as media supplements, nutrients and also cell density.

  • ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) ELISA Kit (ab115343)
    ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) ELISA Kit (ab115343)

    Example control sample curve for HeLa cell extract. The phosphorylation state of PDHA1 can vary by treatment but also by cell culture conditions such as media supplements, nutrients and also cell density.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab115343? Please let us know so that we can cite the reference in this datasheet.

ab115343 has been referenced in 3 publications.

  • Yang Z  et al. Phosphorylated form of pyruvate dehydrogenase a1 mediates tumor necrosis factor a-induced glioma cell migration. Oncol Lett 21:176 (2021). PubMed: 33574915
  • Ahn E  et al. Temporal fluxomics reveals oscillations in TCA cycle flux throughout the mammalian cell cycle. Mol Syst Biol 13:953 (2017). PubMed: 29109155
  • Zhang W  et al. Dicumarol inhibits PDK1 and targets multiple malignant behaviors of ovarian cancer cells. PLoS One 12:e0179672 (2017). PubMed: 28617852

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

I am interested in the detection antibodies used in kits ab115343, ab115344 and ab115345. Can these antibodies be made available separately from the kits?

Read More

Abcam community

Verified customer

Asked on May 20 2014

Answer

Unfortunately we are not able to sell the detection antibodies from this kit individually.

Read More

Jeremy Kasanov

Abcam Scientific Support

Answered on May 20 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.