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SimpleStep

Pre-coated 384 well Microplate SimpleStep ELISA® (ab203359)

  • Datasheet
  • Protocol Booklet
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Comparison of human Frataxin protein standard curve in the 96 well microplate vs 384 well microplate format.
  • Comparison of the human total PSA protein standard curve in the 96 well microplate vs 384 well microplate format.
  • Linearity of dilution of human urine samples measuring total PSA ran in the 384 well microplate.

Key features and details

  • One-wash 90 minute protocol

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Overview

  • Product name

    Pre-coated 384 well Microplate SimpleStep ELISA®
  • Product overview

    Abcam’s 384-Well SimpleStep ELISA® Microplate can be used in place of the 96 well microplate that accompanies any of our 1x96well SimpeStep ELISA® kit from the catalogue.


    To fully utilize all 384-wells, users need to combine reagents from two 1 x 96-well SimpleStep ELISA kits and it is recommended that an automated liquid handling system is used with the 384-well microplate.


    The microplate is pre-coated with the monoclonal antibody of a proprietary tag and is only suitable for use with the our SimpleStep ELISA range.

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 units
    Pre-Coated 384-Well Microplate 1 x 384 units

Images

  • Comparison of human Frataxin protein standard curve in the 96 well microplate vs 384 well microplate format.
    Comparison of human Frataxin protein standard curve in the 96 well microplate vs 384 well microplate format.

    The protein standard from the 96 well microplate human Frataxin SimpleStep ELISA kit (ab176112) was ran on the 384 well microplate. Signal achieved on the 384 well microplate is almost identical that achieved in the 96 well format.

  • Comparison of the human total PSA protein standard curve in the 96 well microplate vs 384 well microplate format.
    Comparison of the human total PSA protein standard curve in the 96 well microplate vs 384 well microplate format.

    The protein standard from the 96 well microplate human total PSA SimpleStep ELISA kit (ab188388) was ran on the 384 well microplate. Signal achieved on the 384 well microplate is almost identical that achieved in the 96 well format.

  • Linearity of dilution of human urine samples measuring total PSA ran in the 384 well microplate.
    Linearity of dilution of human urine samples measuring total PSA ran in the 384 well microplate.

    Total PSA levels were measured in human urine sample, using reagents from the total PSA Human SimpleStep ELISA Kit (ab188388) on the 384 well microplate.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (0)

Publishing research using ab203359? Please let us know so that we can cite the reference in this datasheet.

ab203359 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Great tool

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
All of my previous experiences with ELISA assays were based on 96 well format. Therefore I was a lot suspicious about the possibility and sensitivity to perform in 384 well format. It turned out to be an incredible tool for me to perform ELISA assay without limitations in sample size, decreased amount of reagents and with a great sensitivity.
The only improvement would be to produce those plates as in seperate 16 well strip format, one might not always need all 384 wells but need obviously more than 96 in one set of experiment.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Aug 24 2021

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