For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/elisa/pyruvate-dehydrogenase-pdh-profiling-elisa-kit-ab110174.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Metabolism Energy Metabolism
Share by email

Pyruvate dehydrogenase (PDH) Profiling ELISA Kit (ab110174)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (4)References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

ELISA - Pyruvate dehydrogenase (PDH) Protein Quantity Microplate Assay Kit (ab110174)
  • Sandwich ELISA - Pyruvate dehydrogenase (PDH) Protein Quantity Microplate Assay Kit (ab110174)

Key features and details

  • Sample type: Cell culture extracts, Tissue
  • Detection method: Colorimetric
  • Assay type: Sandwich (qualitative)
  • Reacts with: Mouse, Rat, Cow, Human

You may also be interested in

Assay
Product image
Complex I Enzyme Activity Dipstick Assay Kit (ab109720)
Assay
Product image
Pyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit (ab109882)
Assay
Product image
Pyruvate dehydrogenase (PDH) Enzyme Activity Microplate Assay Kit (ab109902)

View more associated products

Overview

  • Product name

    Pyruvate dehydrogenase (PDH) Profiling ELISA Kit
    See all Pyruvate dehydrogenase kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture extracts, Tissue
  • Assay type

    Sandwich (qualitative)
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human
  • Product overview

    Abcam’s Pyruvate dehydrogenase (PDH) in vitro ProfilingELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the measurement of Pyruvate dehydrogenase (PDH) in Human, bovine, mouse, and rat whole tissue or cell lysate samples.

    Capture antibodies are pre-coated in the wells of modular microplates, which can be broken into 8-well strips. This assay is a “sandwich” ELISA, where the PDH enzyme is purified and immobilized by an anti-PDH capture antibody pre-coated in the microplate wells. The amount of captured PDH is determined by adding a second (detector) anti-PDH antibody which binds to the captured PDH hat a different epitope. This is followed by binding of an HRP conjugated goat anti-mouse antibody that binds the detector anti-PDH antibody. The detector-bound HRP then changes the colorless HRP development solution to blue and the color intensity (absorbance) is proportional to the amount of PDH captured. All of our microplate assays utilize our highly-validated immunocapture antibodies, which are able to capture large, multi-subunit enzyme complexes in their fully intact state.

  • Notes

    5X Stabilizer should be stored at -20°C. Remainder of kit should be stored at 4°C. When stored as recommended the kit is stable for 6 months.

     

    PDH is the key regulatory enzyme of cellular metabolism because it links the TCA cycle and subsequent oxidative phosphorylation with glycolysis and gluconeogenesis as well as with both lipid and amino acid metabolism. PDH activity is regulated primarily by PDK-dependent phosphorylation and PDP-dependent dephosphorylation of PDH. Phosphorylation inactivates PDH whereas dephosphorylation activates PDH. Phosphorylation occurs at Serines 232, 293, and 300 of the human E1α subunits.

  • Platform

    Microplate

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 96 tests
    10X Blocking Solution 1 x 10ml
    20X Buffer 1 x 20ml
    20X Detector Antibody 1 x 1ml
    20X HRP Label 1 x 1ml
    5X Stabilizer 1 x 13ml
    96-well Microplate (12 strips) 1 unit
    Detergent 1 x 1ml
    HRP/TMB Development Solution 1 x 20ml
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
  • Alternative names

    • PDC
    • PDH
    • PDHC

Associated products

  • Positive Controls

    • Bovine Heart Mitochondria (ab110338)
    • Rat liver tissue lysate - mitochondrial extract (ab110346)
    • Rat heart tissue lysate - mitochondrial extract (ab110347)
    • Rat brain tissue lysate - mitochondrial extract (ab110348)
    • Mouse liver tissue lysate - mitochondrial extract (ab110349)
    • Mouse heart tissue lysate - mitochondrial extract (ab110350)
    • Mouse brain tissue lysate - mitochondrial extract (ab110351)

Images

  • ELISA - Pyruvate dehydrogenase (PDH) Protein Quantity Microplate Assay Kit (ab110174)
    ELISA - Pyruvate dehydrogenase (PDH) Protein Quantity Microplate Assay Kit (ab110174)
    An example of the quantity of PDH capture from a HepG2 cultured cell lysate. The sample was diluted to show that over this range of concentrations that can be used. Each sample was measured in 6 replicates. Bars show standard deviations.
  • Sandwich ELISA - Pyruvate dehydrogenase (PDH) Protein Quantity Microplate Assay Kit (ab110174)
    Sandwich ELISA - Pyruvate dehydrogenase (PDH) Protein Quantity Microplate Assay Kit (ab110174)
    Abcams' protein quantity microplate assays use the well-established sandwich ELISA format, whereby capture and detector antibodies are used to immobilize and then quantify a target protein or enzyme. All of our microplate assays utilize our highly-validated immunocapture antibodies, which are able to capture large, multi-subunit enzyme complexes in their fully intact state. Capture antibodies are pre-coated in the wells of premium Nunc MaxiSorp™ modular microplates, which can be broken into 8-well strips. After the target has been immobilized in the well, a second monoclonal antibody, against a different epitope on the target, is added to the well. This detector antibody is either directly labeled with biotin, or a biotin-labeled goat anti-mouse secondary is added. Substrate plus HRP or AP conjugated to streptavidin provide a colorimetric signal that is readable by any plate readers capable of standard ELISA absorbance measurements.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab110174? Please let us know so that we can cite the reference in this datasheet.

ab110174 has been referenced in 2 publications.

  • Zager RA  et al. Renal Cortical Pyruvate Depletion during AKI. J Am Soc Nephrol N/A:N/A (2014). ELISA ; Mouse . PubMed: 24385590
  • Yoshioka J  et al. Deletion of thioredoxin-interacting protein in mice impairs mitochondrial function but protects the myocardium from ischemia-reperfusion injury. J Clin Invest 122:267-79 (2012). PubMed: 22201682

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Question

1) To which subunit does the antibody bind?

2) Does the antibody recognize a linear epitope or a conformational epitope?

3) Does the antibody bind to the active site on enzyme?

Read More

Abcam community

Verified customer

Asked on Dec 23 2013

Answer

To answer your previous questions:

1) To which subunit does the antibody bind?
E2/E3bp

2) Does the antibody recognize a linear epitope or a conformational epitope?
Conformational epitope

3) Does the antibody bind to the active site on enzyme?
The enzyme continues to be active after immunocapture with the antibody.

Read More

Elisa Thomas

Abcam Scientific Support

Answered on Dec 23 2013

Question

1. Under 'Preparation of Samples', Step 4, can the supernatant be stored at -80 degrees C at this point?
2. Under 'Assay Method', Step 2, can the plate be incubated overnight at 4 degrees C instead of at room temp for 3 hrs?

Read More

Abcam community

Verified customer

Asked on Jun 11 2013

Answer

The answer to both questions is YES.

Read More

Abcam Scientific Support

Answered on Jun 11 2013

Question

Needs to quantify the PDH in samples, the only PDH standard available is from pig. Can the antibodies in the kit detect porcine PDH?

Read More

Abcam community

Verified customer

Asked on Aug 27 2012

Answer

Thank you very much for your call last week and for your patience while I have been in touch with the lab about this kit.

The antibodies used in the kit were actually raised against the pig protein,so we expect it to work with the pig protein. Wehave alsoreceived reports from usersstating thatthe kit works with pig, though we have not tested the kit with pig samples in our own lab.

I hope that this informaiton will be useful, but please let me know if you have any further questions and I'll be happy to help.

Read More

Abcam Scientific Support

Answered on Aug 27 2012

Question

I need a kit which will measure preferably enzymatic activity but also could measure protein quantity. My question is, will the 8M urea, 4% CHAPS lysis buffer that my samples are in affect these products?

Read More

Abcam community

Verified customer

Asked on Feb 10 2012

Answer

Thank you for contacting ABcam in regards to the lysis buffer that your tissues are in and how that will work with our PDH kit.

These kits will not function well with samples in such a harsh buffer. It is recommended that samples are in PSB buffers. You may wish to use a buffer exchange system such as a dialysis tubing to remove the 8M urea and replace it with PBS. Thereby enabling you to use these samples in these kits.

I hope that you have found this information useful. Please let me know if you have any questions.

Read More

Abcam Scientific Support

Answered on Feb 10 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.