Rat BDNF ELISA Kit (ab213899)
Key features and details
- Sensitivity: 15 pg/ml
- Range: 31.2 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat BDNF ELISA Kit
See all BDNF kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 88pg/ml 4.4 = 5% 2 16 292pg/ml 16.06 = 5.5% 3 16 906pg/ml 70.66 = 7.8% Inter-assay Sample n Mean SD CV% 1 24 84pg/ml 4.2 = 5% 2 24 273pg/ml 19.11 = 7% 3 24 977pg/ml 77.18 = 7.9% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 15 pg/ml -
Range
31.2 pg/ml - 2000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
The Rat BDNF Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213899) is designed for the quantitative measurement of Rat BDNF in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BDNF has been pre-coated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BDNF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat BDNF/CFH amount of sample captured in plate.
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Notes
Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-rat BDNF coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Rat BDNF antibody 1 x 100µl Lyophilized recombinant Rat BDNF standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
Research areas
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Function
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. -
Tissue specificity
Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta. -
Involvement in disease
Bulimia nervosa 2
Congenital central hypoventilation syndrome -
Sequence similarities
Belongs to the NGF-beta family. -
Post-translational
modificationsThe propeptide is N-glycosylated and glycosulfated.
Converted into mature BDNF by plasmin (PLG). -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Abrineurin
- ANON2
- BDNF
see all -
Database links
- Entrez Gene: 24225 Rat
- SwissProt: P23363 Rat
- Unigene: 11266 Rat
Images
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Rat BDNF ELISA Kit (ab213899) Standard Curve.
Rat BDNF ELISA Kit (ab213899) Standard Curve.
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab213899 has been referenced in 9 publications.
- Wu R et al. Physical exercise promotes integration of grafted cells and functional recovery in an acute stroke rat model. Stem Cell Reports 17:276-288 (2022). PubMed: 35030322
- Al Omairi NE et al. Selenium Nanoparticles with Prodigiosin Rescue Hippocampal Damage Associated with Epileptic Seizures Induced by Pentylenetetrazole in Rats. Biology (Basel) 11:N/A (2022). PubMed: 35336729
- Zhuo X & Jiang H Protective effects of melatonin in cisplatin-induced cardiac toxicity: possible role of BDNF-TNF-a signaling pathway. Acta Cir Bras 37:e370208 (2022). PubMed: 35507972
- Xue J et al. Caffeine improves bladder function in diabetic rats via a neuroprotective effect. Exp Ther Med 21:501 (2021). PubMed: 33791010
- Jiang R & Wei H Beneficial effects of octreotide in alcohol-induced neuropathic pain. Role of H 2S, BDNF, TNF-a and Nrf2. Acta Cir Bras 36:e360408 (2021). PubMed: 34076065
- Callizot N et al. AZP2006, a new promising treatment for Alzheimer's and related diseases. Sci Rep 11:16806 (2021). PubMed: 34413330
- Albrakati A et al. Neuroprotective Efficiency of Prodigiosins Conjugated with Selenium Nanoparticles in Rats Exposed to Chronic Unpredictable Mild Stress is Mediated Through Antioxidative, Anti-Inflammatory, Anti-Apoptotic, and Neuromodulatory Activities. Int J Nanomedicine 16:8447-8464 (2021). PubMed: 35002238
- Endo T et al. Evidence for cell-contact factor involvement in neurite outgrowth of dorsal root ganglion neurons stimulated by Schwann cells. Exp Physiol 104:1447-1454 (2019). PubMed: 31294871
- Liu DW et al. Conditioned taste aversion memory extinction temporally induces insular cortical BDNF release and inhibits neuronal apoptosis. Neuropsychiatr Dis Treat 15:2403-2414 (2019). PubMed: 31933521