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    products/elisa/rat-ckm-elisa-kit-ab187396.pdf

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SimpleStep

Rat CKM ELISA Kit (ab187396)

  • Datasheet
  • SDS
  • Protocol Booklet
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Other - Rat CKM ELISA Kit (ab187396)
  • Example of CKM standard curve in Sample Diluent NS.
  • Example of CKM standard curve in Extraction Buffer PTR.
  • Tissue specificity of CKM in rat tissue extracts, Rat Heart (RHH), brain (RBH) and Skeletal Muscle (RSkMH).
  • Serum species reactivity.
  • Serum species reactivity.

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 5.83 ng/ml
  • Range: 8.8 ng/ml - 100 ng/ml
  • Sample type: Cit plasma, Serum, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Rat

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Overview

  • Product name

    Rat CKM ELISA Kit
    See all Creatine Kinase MM kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 4%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 5%
  • Sample type

    Serum, Tissue Extracts, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    5.83 ng/ml
  • Range

    8.8 ng/ml - 100 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 94 89% - 97%
    Extraction Buffer 96 87% - 101%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Rat
  • Product overview

    Rat CKM ELISA Kit (ab187396) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CKM protein in serum, tissue extracts, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat CKM with 5.83 ng/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Creatine kinase (CK), also known as creatine phosphokinase (CPK) or phospho-creatine kinase, is an enzyme that catalyzes the reversible transfer of a high-energy phosphate group between phosphocreatine and ATP. There are five known CK isozymes, three located in the cytosol (CKMM, CKMB, CKBB) and two in the mitochondria (MiSCK and MiuCK). Structurally, cytosolic CK is a 86 kDa dimeric enzyme of two identical or non-identical chains, CKM and/or CKB whereas mitochondrial CK is an octameric enzyme associated with mitochondrial membranes. The compartmentation of CK isoenzymes allows for direct association with ATP-providing or consuming processes both linked via metabolite channeling through the creatine phosphate shuttle. Thus CK isoenzymes allow for the generation of a large pool of phosphocreatine, considered to be a temporal and spatial ATP buffering system in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

    Expression of cytosolic isoenzyme patterns differ between tissues, with skeletal muscle expressing high levels of CK-MM (98%) and low levels of CK-MB (1%), the myocardium (heart muscle) expressing CK-MM at 70% and CK-MB at 25–30% and the brain and neural tissue expressing mainly CK-BB. CKM is also found in serum from normal animals and levels vary based on muscle mass, age, physical activity and underlying pathologies.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    10X CKM Capture Antibody 1 x 600µl
    10X CKM Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BI 1 x 6ml
    Plate Seals 1 unit
    Rat CKM Lyophilized Purified Protein 2 vials
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Signal transduction proteins ELISA kits
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Neuroscience
    • Diseases
  • Cellular localization

    Cytoplasmic
  • Alternative names

    • CKM
    • CKMM
    • creatine kinase M chain
    • creatine kinase M-type
    • creatine kinase, muscle
    • M-CK
    see all
  • Database links

    • Entrez Gene: 24265 Rat
    • SwissProt: P00564 Rat

    Images

    • Other - Rat CKM ELISA Kit (ab187396)
      Other - Rat CKM ELISA Kit (ab187396)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of CKM standard curve in Sample Diluent NS.
      Example of CKM standard curve in Sample Diluent NS.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Example of CKM standard curve in Extraction Buffer PTR.
      Example of CKM standard curve in Extraction Buffer PTR.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Tissue specificity of CKM in rat tissue extracts, Rat Heart (RHH), brain (RBH) and Skeletal Muscle (RSkMH).
      Tissue specificity of CKM in rat tissue extracts, Rat Heart (RHH), brain (RBH) and Skeletal Muscle (RSkMH).

      Levels of CKM in 10 µg/mL extract were interpolated from a standard curve in 1X Cell Extraction Buffer PTR.

    • Serum species reactivity.
      Serum species reactivity.

      Canine, Guinea pig, Porcine and Rabbit serum was loaded in the assay at 2.5% in Sample Diluent NS. Absorbance levels after background subtraction are displayed.

    • Serum species reactivity.
      Serum species reactivity.

      Human, Mouse and Goat serum was loaded in the assay at 1% in Sample Diluent NS. Absorbance levels after background subtraction are displayed.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (5)

    Publishing research using ab187396? Please let us know so that we can cite the reference in this datasheet.

    ab187396 has been referenced in 5 publications.

    • Mohamed ME  et al. Cardioprotective Effect of Linalool against Isoproterenol-Induced Myocardial Infarction. Life (Basel) 11:N/A (2021). PubMed: 33562483
    • Sun C  et al. Atractylenolide I alleviates ischemia/reperfusion injury by preserving mitochondrial function and inhibiting caspase-3 activity. J Int Med Res 49:300060521993315 (2021). PubMed: 33641489
    • Younis NS  et al. Protective Effect of Geraniol on Oxidative, Inflammatory and Apoptotic Alterations in Isoproterenol-Induced Cardiotoxicity: Role of the Keap1/Nrf2/HO-1 and PI3K/Akt/mTOR Pathways. Antioxidants (Basel) 9:N/A (2020). PubMed: 33053761
    • Moreira JB  et al. A small molecule activator of AKT does not reduce ischemic injury of the rat heart. J Transl Med 13:76 (2015). Sandwich ELISA ; Rat . PubMed: 25889299
    • Holwerda AM & Locke M Hsp25 and Hsp72 content in rat skeletal muscle following controlled shortening and lengthening contractions. Appl Physiol Nutr Metab 39:1380-7 (2014). ELISA ; Rat . PubMed: 25356915

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