Rat IL-1RA Antibody Pair - BSA and Azide free (ab253848)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Rat
Product nameRat IL-1RA Antibody Pair - BSA and Azide free
See all IL-1RA kits
Assay typeELISA set
Species reactivityReacts with: Rat
Rat IL-1RA is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Rat IL-1RA in sandwich ELISAs and many other pair-based applications.
The pair can be used in variety of assays and platforms including but not limited to:
-Meso Scale Discovery® (MSD®)
-Simoa® and Single Molecule Counting (SMC™) immunoassays
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
-Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
-Concentration of ~1 mg/ml as measured by the protein A280 method.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for biotin, HRP, gold, and fluorescent dyes.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration: ~1 mg/ml
Storage buffer: 100% PBS
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
For more information see here.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.
Tested applicationsSuitable for: Sandwich ELISAmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 10 x 96 tests Rat IL-1Ra (IL1RN) Capture Antibody (unconjugated) 1 x 100µg Rat IL-1Ra (IL1RN) Detector Antibody (unconjugated) 1 x 100µg
FunctionInhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
Tissue specificityThe intracellular form of IL1RN is predominantly expressed in epithelial cells.
Involvement in diseaseMicrovascular complications of diabetes 4
Interleukin 1 receptor antagonist deficiency
Cellular localizationCytoplasm and Secreted.
- Information by UniProt
- ICIL 1RA
- Entrez Gene: 60582 Rat
- SwissProt: P25086 Rat
- Unigene: 162640 Rat
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab253848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use at an assay dependent concentration.
To learn more about the advantages of recombinant antibodies see here.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
ab253848 has not yet been referenced specifically in any publications.