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    products/elisa/rat-pai1-elisa-kit-serpine1-ab201283.pdf

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Cardiovascular Blood Fibrinolysis / Thrombolysis
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RecombinantSimpleStep

Rat PAI1 ELISA Kit (SERPINE1) (ab201283)

  • Datasheet
  • SDS
  • Protocol Booklet
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Other - Rat PAI1 ELISA Kit (SERPINE1) (ab201283)
  • Example of PAI1 standard curve.
  • Interpolated concentrations of PAI1 in rat serum.
  • Interpolated Concentration of PAI1 in rat serum, plasma-citrate, and urine.
  • Sandwich ELISA - Rat PAI1 ELISA Kit (SERPINE1) (ab201283)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 159 pg/ml
  • Range: 298 pg/ml - 5000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse, Rat

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Overview

  • Product name

    Rat PAI1 ELISA Kit (SERPINE1)
    See all PAI1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    rat serum 5 1.6%
    Inter-assay
    Sample n Mean SD CV%
    rat serum 3 3.3%
  • Sample type

    Cell culture supernatant, Urine, Serum, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    159 pg/ml
  • Range

    298 pg/ml - 5000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 80 69% - 88%
    Serum 100 94% - 105%
    Cell culture media 99 98% - 102%
    Cit plasma 85 84% - 87%
    serum free media 95 91% - 98%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse, Rat
  • Product overview

    Rat PAI1 ELISA Kit (SERPINE1) (ab201283) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PAI1 (SERPINE1) protein in cell culture supernatant, cit plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat PAI1 (SERPINE1) with 159 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Plasminogen activator inhibitor-1 (PAI1 or Serpin E1) is encoded by the Serpine1 gene. PAI1 is a serine protease inhibitor with a major role in the regulation of fibrinolysis. PAI1 is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), both of which are activators of plasminogen. PAI1 is a secreted protein produced by the endothelium as well as adipose tissue.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Rat PAI1 Capture Antibody 1 x 600µl
    10X Rat PAI1 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BI 1 x 6ml
    Plate Seals 1 unit
    Rat PAI1 Lyophilized Recombinant Protein 2 vials
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Cardiovascular
    • Blood
    • Fibrinolysis / Thrombolysis
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Protease inhibitors
    • Serine protease inhibitors
    • SERPINs
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Protease inhibitors
    • Other protease inhibitors
    • Cancer
    • Tumor biomarkers
    • Other
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Protease inhibitors ELISA kits
    • Metabolism
    • Types of disease
    • Metabolic disorders
  • Function

    This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
  • Tissue specificity

    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • Involvement in disease

    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • Sequence similarities

    Belongs to the serpin family.
  • Post-translational
    modifications

    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P05121 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Clade E
    • Endothelial plasminogen activator inhibitor
    • Nexin
    • Nexin plasminogen activator inhibitor type 1
    • PAI
    • PAI 1
    • PAI-1
    • PAI1_HUMAN
    • PLANH1
    • Plasminogen activator inhibitor 1
    • Plasminogen activator inhibitor type 1
    • Serine (or cysteine) proteinase inhibitor
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • Serine proteinase inhibitor clade E member 1
    • serpin
    • Serpin E1
    • Serpin peptidase inhibitor clade E
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • Serpine 1
    • SERPINE1
    see all
  • Database links

    • Entrez Gene: 18787 Mouse
    • Entrez Gene: 24617 Rat
    • SwissProt: P22777 Mouse
    • SwissProt: P20961 Rat
    • Unigene: 250422 Mouse
    • Unigene: 29367 Rat

    Associated products

    • Alternative Versions

      • Rat PAI1 Antibody Pair - BSA and Azide free (SERPINE1) (ab243984)
    • Related Products

      • Mouse PAI1 ELISA Kit (SERPINE1) (ab197752)

    Images

    • Other - Rat PAI1 ELISA Kit (SERPINE1) (ab201283)
      Other - Rat PAI1 ELISA Kit (SERPINE1) (ab201283)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of PAI1 standard curve.
      Example of PAI1 standard curve.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of PAI1 in rat serum.
      Interpolated concentrations of PAI1 in rat serum.

      The concentrations of PAI1 were measured in duplicate and interpolated from the PAI1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAI1 concentration was determined to be 5,897 pg/mL in mouse serum.

    • Interpolated Concentration of PAI1 in rat serum, plasma-citrate, and urine.
      Interpolated Concentration of PAI1 in rat serum, plasma-citrate, and urine.

      The concentrations of PAI1 were measured in duplicate and interpolated from the PAI1 standard curve and corrected for sample dilution. The Interpolated dilution factor corrected values are plotted (mean+/- SD, n=2).

    • Sandwich ELISA - Rat PAI1 ELISA Kit (SERPINE1) (ab201283)
      Sandwich ELISA - Rat PAI1 ELISA Kit (SERPINE1) (ab201283)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab201283? Please let us know so that we can cite the reference in this datasheet.

    ab201283 has been referenced in 2 publications.

    • Wong RMY  et al. Fibrinolysis as a target to enhance osteoporotic fracture healing by vibration therapy in a metaphyseal fracture model. Bone Joint Res 10:41-50 (2021). PubMed: 33448865
    • Ito Y  et al. Generation and characterization of tissue-type plasminogen activator transgenic rats. J Thromb Thrombolysis 45:77-87 (2018). PubMed: 29168147

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