Rat TNF alpha ELISA Kit (ab100784)
Key features and details
- Sensitivity: 25 pg/ml
- Range: 82.3 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat TNF alpha ELISA Kit
See all TNF alpha kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 25 pg/ml -
Range
82.3 pg/ml - 20000 pg/ml -
Recovery
96 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 101.54 89% - 108% Serum 95.43 84% - 104% Plasma 93.79 82% - 102% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Abcam’s TNF alpha Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat TNF alpha in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Rat TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Rat TNF alpha 2 vials Recombinant Rat TNF alpha Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml TNF alpha Microplate (12 x 8 wells) 1 unit -
Research areas
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Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
Involvement in disease
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- APC1
- APC1 protein
- Cachectin
see all -
Database links
- Entrez Gene: 24835 Rat
- SwissProt: P16599 Rat
- Unigene: 2275 Rat
Images
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ELISA: TNF alpha Rat ELISA Kit (ab100784)
Rat TNF alpha measured in culture supernatants (dilution range 1/1-1/200) from control NR8383 cells or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) and PMA with the addition of 1 ug x mL-1 of LPS (Sigma) for the last 6 hours (duplicates +/- SD).
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Typical Standard CurveRepresentative Standard Curve using ab100784.
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Typical Standard CurveRepresentative Standard Curve using ab100784.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab100784 has been referenced in 6 publications.
- Potter HG et al. Maternal behaviours and adult offspring behavioural deficits are predicted by maternal TNFα concentration in a rat model of neurodevelopmental disorders. Brain Behav Immun 108:162-175 (2023). PubMed: 36503051
- Kowash HM et al. Maternal immune activation in rats induces dysfunction of placental leucine transport and alters fetal brain growth. Clin Sci (Lond) 136:1117-1137 (2022). PubMed: 35852150
- Nusair SD & Ahmad MI Toxicity of Vipera palaestinae venom and antagonistic effects of methanolic leaf extract of Eryngium creticum lam. Toxicon 166:1-8 (2019). PubMed: 31095960
- Liu Z et al. Number 2 Feibi Recipe Reduces PM2.5-Induced Lung Injury in Rats. Evid Based Complement Alternat Med 2018:3674145 (2018). PubMed: 29541141
- Zhang C & Liu YJ Biomechanic and histologic analysis of fibroblastic effects of tendon-to-bone healing by transforming growth factor ß1 (TGF-ß1) in rotator cuff tears. Acta Cir Bras 32:1045-1055 (2017). PubMed: 29319733
- Akinrinmade FJ et al. Changes in serum cytokine levels, hepatic and intestinal morphology in aflatoxin B1-induced injury: modulatory roles of melatonin and flavonoid-rich fractions from Chromolena odorata. Mycotoxin Res 32:53-60 (2016). PubMed: 26798045