Rat TNF alpha ELISA Kit (ab46070)
Key features and details
- Sensitivity: 15 pg/ml
- Range: 31.25 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat TNF alpha ELISA Kit
See all TNF alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% A 12 543.5pg/ml 44.7 8.2% B 10 130.1pg/ml 10.3 7.9% Inter-assay Sample n Mean SD CV% A 9 541.4pg/ml 33.2 6.1% B 9 149.4pg/ml 9.8 6.5% -
Sample type
Cell culture supernatant, Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
< 15 pg/ml -
Range
31.25 pg/ml - 1000 pg/ml -
Assay time
3h 45m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Abcam’s TNF alpha Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Rat TNF alpha in cell culture supernatants, serum and plasma samples.
A monoclonal antibody specific for TNF alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TNF alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TNF alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TNF alpha present in the samples.
This kit will recognize both endogenous and recombinant rat TNF alpha
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 2 x 96 tests 1 x 96 tests 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 15ml 1 x 15ml 200X Wash Buffer White 2 x 10ml 1 x 10ml 1 x 10ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7.5ml 1 x 7.5ml Biotinylated anti-TNF alpha Red 2 x 400µl 1 x 400µl 1 x 400µl Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml 1 x 11ml HRP Diluent Red 1 x 23ml 1 x 12ml 1 x 12ml Plastic Plate Covers 4 units 2 units 2 units Stop Reagent Black 2 x 11ml 1 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl 2 x 5µl TNF alpha Microplate (12 x 8 well strips) 2 units 1 unit 1 unit TNF alpha Standard (Lyophilized) Yellow 4 vials 2 vials 2 vials -
Research areas
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Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
Involvement in disease
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- APC1
- APC1 protein
- Cachectin
see all -
Database links
- Entrez Gene: 24835 Rat
- SwissProt: P16599 Rat
- Unigene: 2275 Rat
Associated products
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SimpleStep ELISA kits
Images
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ELISA: TNF alpha Rat ELISA Kit (ab46070)
Rat TNF alpha measured in culture supernatants (dilution range 1/1-1/200) from control NR8383 cells or cells stimulated for 24 hours with 50 ng x mL-1 of PMA ((PMA) ab120297) or PMA with the addition of 1 ug x mL-1 of LPS (Sigma) (LPS+PMA) for the last 6 hours (duplicates +/- SD).
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ELISA: TNF alpha Rat ELISA Kit (ab46070)Standard curve with background signal subtracted (duplicates; +/- SD).
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Typical Standard CurveRepresentative Standard Curve using ab46070
Datasheets and documents
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SDS download
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Datasheet download
References (96)
ab46070 has been referenced in 96 publications.
- Li T et al. Ulinastatin Improves Renal Microcirculation by Protecting Endothelial Cells and Inhibiting Autophagy in a Septic Rat Model. Kidney Blood Press Res 47:256-269 (2022). PubMed: 35016182
- Alsabaani NA et al. Maslinic Acid Protects against Streptozotocin-Induced Diabetic Retinopathy by Activating Nrf2 and Suppressing NF-κB. J Ophthalmol 2022:3044202 (2022). PubMed: 35265366
- Borrego I et al. Fibrin, Bone Marrow Cells and Macrophages Interactively Modulate Cardiomyoblast Fate. Biomedicines 10:N/A (2022). PubMed: 35327330
- Zhuo X & Jiang H Protective effects of melatonin in cisplatin-induced cardiac toxicity: possible role of BDNF-TNF-a signaling pathway. Acta Cir Bras 37:e370208 (2022). PubMed: 35507972
- Younis NS ß-Caryophyllene Ameliorates Cyclophosphamide Induced Cardiac Injury: The Association of TLR4/NF?B and Nrf2/HO1/NQO1 Pathways. J Cardiovasc Dev Dis 9:N/A (2022). PubMed: 35621844