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Rat TNF alpha ELISA Set (without plates) (ab48419)

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TNFa Rat ELISA Set (ab48419)

    Overview

    • Product name

      Rat TNF alpha ELISA Set (without plates)
      See all TNF alpha kits

    • Sample type

      Cell culture supernatant, Serum, Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      < 15 pg/ml

    • Range

      31.25 pg/ml - 1000 pg/ml

    • Assay duration

      Multiple steps standard assay

    • Species reactivity

      Reacts with: Rat

    • Product overview

      Abcam’s TNFα Rat in vitro ELISA Set is designed for the quantitative measurement of Rat TNFα in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.


      The rat TNF alpha ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.


      This ELISA Set is intended to measure rat TNF alpha in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.


      Recognizes both natural and recombinant Rat TNF alpha Range: 31.25 pg/ml - 1000 pg/ml Sensitivity < 15 pg/ml

    • Tested applications

      Suitable for: Sandwich ELISAmore details

    • Platform

      Reagents

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components Identifier 5 x 96 tests 10 x 96 tests 15 x 96 tests
      Detection biotinylated anti-rat TNF alpha antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml
      Rat TNF alpha Capture antiboy 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml
      TNF alpha Standard (Lyophilized) Yellow 5 vials 10 vials 15 vials
      Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials
      Streptavidin-HRP 1 vial 2 vials 3 vials

    • Research areas

    • Function

      Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

    • Involvement in disease

      Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).

    • Sequence similarities

      Belongs to the tumor necrosis factor family.

    • Post-translational
      modifications

      The soluble form derives from the membrane form by proteolytic processing.
      The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
      O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt

    • Alternative names

      • APC1
      • APC1 protein
      • Cachectin
      • DIF
      • Differentiation inducing factor
      • Macrophage cytotoxic factor
      • Tnf
      • TNF superfamily member 2
      • TNF superfamily, member 2
      • TNF, macrophage derived
      • TNF, monocyte derived
      • TNF-a
      • TNF-alpha
      • TNFA
      • TNFA_HUMAN
      • TNFSF2
      • Tumor necrosis factor
      • Tumor necrosis factor (TNF superfamily member 2)
      • Tumor necrosis factor alpha
      • Tumor necrosis factor ligand superfamily member 2
      • Tumor Necrosis Factor, Membrane Form
      • Tumor necrosis factor, soluble form
      see all

    • Database links

      Applications

      The Abpromise guarantee

      Our Abpromise guarantee covers the use of ab48419 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Abreviews Notes
      Sandwich ELISA
      Use at an assay dependent concentration.
      Notes
      Sandwich ELISA
      Use at an assay dependent concentration.

      Images

      • TNFa Rat ELISA Set (ab48419)
        TNFa Rat ELISA Set (ab48419)

        Representative Standard Curve using ab48419.

      References (0)

      Publishing research using ab48419? Please let us know so that we can cite the reference in this datasheet.

      ab48419 has not yet been referenced specifically in any publications.

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