Key features and details
- One-wash 90 minute protocol
Product nameSimpleStep ELISA® Capture Peptide Conjugation Kit
SimpleStep ELISA® Capture Peptide Conjugation Kit (ab270690) allows the capture peptide conjugation to be set up in less than 30 seconds, simply by adding a solution of the antibody to a lyophilized mixture containing a proprietary activated peptide.
By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, this process can be used to label both small (e.g. 20 μg) and large quantities of primary antibodies with ease. Batch-to-batch variation upon scale-up is minimal as the process is so simple, and recoveries are always 100%.
It can also be used to label proteins, peptides and other biomolecules.
Capture antibodies tagged with capture peptide are paired with detection antibodies labeled with HRP to be then used within SimpleStep ELISA® Custom ELISA Kit (ab270552). Upon addition to the Assay Plate, the non-biological capture peptide is bound with high affinity to the antibody, pre-coated on the plates, and the immuno-complex is thus immobilized into the well.
This product is manufactured by Expedeon, an Abcam company. It was previously called Lightning-Link® CaptSure™ Peptide conjugation kit. Expedeon product code 300007 is the same as the 1 kit size of this product.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 5 x 96 tests 5 x 96 tests Capture Modifier reagent (ab270690) 1 x 100µl 1 x 100µl Capture Peptide (ab270690) 3 x 100µg 3 x 100µg Capture Quencher reagent (ab270690) 1 x 100µl 1 x 100µl
ab270690 has not yet been referenced specifically in any publications.