DoubleStain IHC Kit: M&R on human tissue (HRP/Green & AP/Red) (ab210061)
Overview
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Product name
DoubleStain IHC Kit: M&R on human tissue (HRP/Green & AP/Red) -
Sample type
Tissue -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s DoubleStain IHC Kit: M&R on human tissue (HRP/Green & AP/Red) (ab210061) is designed to detect two distinct antigens on human tissue or cell samples using a simplified protocol with user-supplied mouse and rabbit antibodies. This kit has been tested in paraffin embedded tissues. However, this kit can be used to stain frozen specimen and/or freshly prepared monolayer cell smears.
Double staining is a common method used in immunohistochemistry to evaluate two distinct antigens in a single tissue. This kit contains a HRP-Polymer anti-Mouse IgG, AP-Polymer anti-Rabbit IgG, and two distinct chromogens, Emerald (green) and Permanent Red (red). User may apply the two enzyme conjugates onto the specimen at the same time and mix them on the slide. Simplified steps offer user much faster and quicker protocol than a sequential procedure. A second advantage is it allows the researcher to visualize when two proteins are co-localized because of the color change when the chromogens overlap that can be semi-quantitative. For example, if the area of co-localization stains blue, the antigen indicated by Emerald is expressed at higher concentration in the cell and if the color is purple, the antigen indicated by Permanent-Red is expressed at higher concentrations DoubleStain IHC Kit: M&R on human tissue (HRP/Green &AP/Red) is a non-biotin system that avoids endogenous biotin non-specific binding.
The Organic Mounting Medium is not supplied with the 120 mL kit.
The Permanent Red working solution is not stable. It must be used (freshly) immediately after it is made.
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Tested applications
Suitable for: IHCmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 12 ml 36 ml 120 ml Emerald Chromogen 1 x 15ml 2 x 18ml 1 x 120ml Mouse HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Organic Mounting Medium 1 x 12ml 4 x 9ml 0 x 0 unit Permanent Red Activator (5X) 1 x 3ml 1 x 7.2ml 2 x 12ml Permanent Red Chromogen (100X) 1 x 150µl 1 x 360µl 1 x 1.2ml Permanent Red Substrate (RTU) 1 x 15ml 2 x 18ml 1 x 120ml Rabbit AP polymer 1 x 6ml 1 x 18ml 1 x 60ml
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC
Use at an assay dependent concentration. |
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab210061 has been referenced in 3 publications.
- Sandoval Pacheco CM et al. Macrophage Polarization in the Skin Lesion Caused by Neotropical Species of Leishmania sp. J Immunol Res 2021:5596876 (2021). PubMed: 33937417
- Mehdi SJ et al. Mesenchymal stem cells gene signature in high-risk myeloma bone marrow linked to suppression of distinct IGFBP2-expressing small adipocytes. Br J Haematol 184:578-593 (2019). PubMed: 30408155
- Nimmakayala RK et al. Cigarette Smoke Induces Stem Cell Features of Pancreatic Cancer Cells via PAF1. Gastroenterology 155:892-908.e6 (2018). PubMed: 29864419