DoubleStain IHC Kit: R&Rt on human/mouse tissue (Green/HRP & AP/Red) (ab183285)
Overview
-
Product name
DoubleStain IHC Kit: R&Rt on human/mouse tissue (Green/HRP & AP/Red) -
Assay type
Polymer Multiplex -
Product overview
Abcam's Doublestain IHC Kit (ab183285) is designed for use with user supplied rabbit and rat primary antibodies to detect two distinct antigens on human and mouse tissue or cell samples. This kit has been tested on paraffin embedded tissue, however, it can also be used to stain frozen specimens or freshly prepared monolayer cell smears.
Double staining is a common method used in immunohistostaining, allowing for the detection of two distinct antigens in a single tissue. Abcam's Doublestain IHC Kit (ab183285) supplies the user with two polymer enzyme conjugates: Rat HRP Polymer (minimal cross reaction to mouse) and Rabbit AP Polymer with two distinct substrates/chromogens, Emerald and Permanent Red. Emerald chromogen reacts with the Rat HRP polymer conjugate to produce a green color. Permanent Red reacts with the Rabbit AP polymer to produce a red color. When two proteins are co-localized a blue/ purple color will develop depending on which antigen is stronger. If only the rat antigen is present only the Emerald chromogen will be present and if the rabbit antigen is present only the Permanent Red chromogen will be present. Abcam's Doublestain IHC Kit (ab183285) is a non-biotin system avoiding the extra steps involved in blocking non-specific binding due to endogenous biotin.
-
Tested applications
Suitable for: IHC-Fr, IHC-Pmore details
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 12 ml 36 ml 120 ml Emerald Chromogen 1 x 15ml 2 x 18ml 1 x 120ml Non-aqueous Mounting Medium 1 x 12ml 4 x 9ml 0 x 0ml Permanent Red Activator (5x) 1 x 3ml 1 x 7.2ml 2 x 12ml Permanent Red Chromogen (100x) 1 x 150µl 1 x 360µl 1 x 1.2ml Permanent Red Substrate 1 x 15ml 2 x 18ml 1 x 120ml Rabbit AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Rat HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab183285 has been referenced in 2 publications.
- Leal AS et al. Retinoid X receptor agonist LG100268 modulates the immune microenvironment in preclinical breast cancer models. NPJ Breast Cancer 5:39 (2019). PubMed: 31700995
- Bathini P et al. Progressive signaling changes in the olfactory nerve of patients with Alzheimer's disease. Neurobiol Aging 76:80-95 (2019). PubMed: 30708185