Mouse and Rabbit Specific HRP (ABC) Detection IHC Kit (ab93677)
Overview
-
Product name
Mouse and Rabbit Specific HRP (ABC) Detection IHC Kit
See all Polyvalent HRP kits -
Product overview
Mouse and Rabbit Specific HRP (ABC) Detection IHC Kit ab93677 contains complet reagents for a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.
Reacts with Mouse and Rabbit IgG (H+L)
-
Notes
ab93677 does not contain chromogen or substrate.
-
Tested applications
Suitable for: IHC-Pmore details
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 60 ml Biotinylated Goat Anti-Polyvalent 1 x 60ml Protein block 1 x 60ml Streptavidin Peroxidase 1 x 60ml -
Research areas
-
Relevance
Polyvalent HRP is a labeled streptavidin-biotin immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates. -
Alternative names
- Polyvalent/HRP
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93677 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent dilution.
|
| Notes |
|---|
|
IHC-P
Use at an assay dependent dilution. |
Images
-
Anti-Mouse and Rabbit Specific HRP (ABC) Detection IHC Kit
Immunohistochemical analysis of Human Tonsil tissue labeling Ki-67 using the ab93677 anti-Mouse and Rabbit specific HRP (ABC) Detection IHC kit.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (10)
ab93677 has been referenced in 10 publications.
- Gheware A et al. ACE2 protein expression in lung tissues of severe COVID-19 infection. Sci Rep 12:4058 (2022). PubMed: 35260724
- Porcellato I et al. Comparison of immunohistochemical and qPCR methods from granulomatous dermatitis lesions for detection of leishmania in dogs living in endemic areas: a preliminary study. Parasit Vectors 15:104 (2022). PubMed: 35331318
- Sforna M et al. Characterization of Primary Cultures of Normal and Neoplastic Canine Melanocytes. Animals (Basel) 11:N/A (2021). PubMed: 33802040
- Silvestri S et al. E-Cadherin Expression in Canine Melanocytic Tumors: Histological, Immunohistochemical, and Survival Analysis. Vet Pathol 57:608-619 (2020). PubMed: 32578507
- Porcellato I et al. Tumour-infiltrating lymphocytes in canine melanocytic tumours: An investigation on the prognostic role of CD3+ and CD20+ lymphocytic populations. Vet Comp Oncol 18:370-380 (2020). PubMed: 31750993
- Lopes-Coelho F et al. Monocytes as Endothelial Progenitor Cells (EPCs), Another Brick in the Wall to Disentangle Tumor Angiogenesis. Cells 9:N/A (2020). PubMed: 31906296
- Aka E & Eren U Distribution of TLR4 and MHC class II molecules of the spleen in broiler chicks treated with and without LPS in the first 2 weeks of the post-hatch period. Br Poult Sci 60:130-138 (2019). PubMed: 30646748
- Yildiz M et al. Histological and immunohistochemical studies of the proximal caecum and caecal tonsils of quail (Coturnix coturnix japonica). Anat Histol Embryol 48:476-485 (2019). PubMed: 31305954
- Aydogan A & Özmen Ö Expression of nectin-4 as a potential biomarker in enzootic nasal adenocarcinoma of goats. Acta Vet Hung 64:71-7 (2016). PubMed: 26919144
- Aydogan A et al. Immunohistochemical expression of caspase-3, caspase-5, caspase-7 and apoptotic protease-activating factor-1 (APAF-1) in the liver and kidney of rats exposed to zoledronic acid (ZOL) and basic fibroblast growth factor (bFGF). Vet Q 34:137-42 (2014). PubMed: 24865775