Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready To Use) (ab93697)
Overview
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Product name
Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready To Use)
See all Polyvalent HRP kits -
Product overview
The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system with increased sensitivity. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.
ab93697 does not contain chromogen or substrate.
Reacts with Mouse and Rabbit IgG (H+L).
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Tested applications
Suitable for: IHC-Pmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 125 ml 60 ml Biotinylated Goat Anti-Polyvalent Plus 1 x 125ml 1 x 60ml Protein block 1 x 125ml 1 x 60ml Streptavidin Peroxidase Plus 1 x 125ml 1 x 60ml -
Research areas
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Relevance
Polyvalent HRP is a labeled streptavidin-biotin immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates. -
Alternative names
- Polyvalent/HRP
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent dilution.
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| Notes |
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IHC-P
Use at an assay dependent dilution. |
Images
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Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready To Use)Image from McGee-Lawrence ME et al., Biochem Biophys Res Commun. 2013;411(4):886-90. Fig 3.; doi: 10.1016/j.bbrc.2013.10.155 with permission from Elsevier.
Immunohistochemistry analysis of mouse femur tissue sections. Femurs were fixed in 10% neutral buffered formalin and decalcified for 7 days in 15% EDTA; complete decalcification was confirmed by X-ray. Decalcified bones were embedded in paraffin and sectioned longitudinally through the defect at a thickness of 8 microns. Immunohistochemical staining was performed with monoclonal anti-activated beta-catenin (un-phosphorylated on Ser33/37 and Thr41), or an IgG isotype control. Chromogens were developed using ab93697, Mouse and Rabbit Specific HRP Plus (ABC) Detection IHC Kit (Ready to Use), followed by 3′-3′-diaminobenzidine (DAB), and then counterstained with fast green. Beta-catenin levels are elevated in defects of Sost-/- mice.
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab93697 has been referenced in 20 publications.
- Azadegan-Dehkordi F et al. Increased Indoleamine 2, 3-Dioxygenase expression modulates Th1/Th17/Th22 and Treg pathway in humans with Helicobacter Pylori-Infected gastric mucosa. Hum Immunol 82:46-53 (2021). PubMed: 33127161
- Pietrzyk L et al. Clinical Value of Detecting Tumor Endothelial Marker 8 (ANTXR1) as a Biomarker in the Diagnosis and Prognosis of Colorectal Cancer. Cancer Manag Res 13:3113-3122 (2021). PubMed: 33859497
- Kaymaz A et al. Investigating the effects of carvacrol in rats using oxygen-induced retinopathy model. Indian J Ophthalmol 69:1219-1223 (2021). PubMed: 33913864
- Weaver SR et al. Pleckstrin homology (PH) domain and Leucine Rich Repeat Phosphatase 1 (Phlpp1) Suppresses Parathyroid Hormone Receptor 1 (Pth1r) Expression and Signaling During Bone Growth. J Bone Miner Res 36:986-999 (2021). PubMed: 33434347
- Farias TO et al. Male reproductive morphofunctional evaluation of a Neotropical sperm-storing vespertilionid bat (Myotis levis) in an environmental context. Cell Tissue Res 382:639-656 (2020). PubMed: 32686003