Mouse and Rabbit Specific HRP/AEC (ABC) Detection IHC Kit (ab93705)
Overview
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Product name
Mouse and Rabbit Specific HRP/AEC (ABC) Detection IHC Kit
See all HRP/AEC Detection Kit kits -
Product overview
Mouse and Rabbit specific HRP/AEC (ABC) IHC Detection Kit ab93705 is a complete kit to detect a primary antibody raised in either mouse or rabbit, using HRP-labeled-streptavidin and a biotinylated anti-rabbit/anti-mouse secondary antibody.
It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and AEC substrate.
In the method:
- a sample is incubated with an unconjugated primary antibody specific to the target antigen
- a biotinylated secondary antibody then binds to the primary antibody
- HRP-labeled streptavidin then binds to the secondary antibody
- the HRP produces a colored substance at the site of primary antibody binding by reacting with AEC. -
Notes
For alternative AEC streptavidin-biotin IHC detection kits see:
- anti-Rabbit HRP-AEC IHC kit ab64260
- Anti-Mouse HRP-AEC IHC Kit ab64258For high sensitivity AEC HRP micro-polymer IHC detection kits see:
- Anti-Mouse / anti-Rabbit HRP-AEC Micro-polymer IHC Kit ab236467
- Anti-Rabbit HRP-AEC Micro-polymer IHC Kit ab236468
Alternatively, see HRP-DAB IHC detection kits.Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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Tested applications
Suitable for: IHC-Pmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 15 ml AEC Single Solution 1 x 15ml Biotinylated Goat Anti-Polyvalent 1 x 15ml Hydrogen Peroxide Block 1 x 15ml Protein block 1 x 15ml Streptavidin Peroxidase 1 x 15ml -
Research areas
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Relevance
An HRP/AEC Detection Kit is a labeled immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates. AEC is 3-amino-9-ethyl-carbazole, a chromogenic substrate. -
Alternative names
- carbazole
- Horseradish Peroxidase
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93705 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent dilution.
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| Notes |
|---|
|
IHC-P
Use at an assay dependent dilution. |
Images
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CD34 detection using HRP/AEC Detection Kit (ab93705)This image is courtesy of an Abreview submitted by Stanislava Reinstein Merjava
Human cornea (cryosections fixed in cold acetone) stained with anti-CD34 antibody using ab93705 Mouse and Rabbit Specific HRP/AEC detection IHC kit.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/AEC detection IHC kit (ab93705)Human tonsil stained with anti-CD3 antibody using ab93705 Polyvalent HRP/AEC Detection Kit (Ready-To-Use).
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab93705 has been referenced in 14 publications.
- Toney NJ et al. Tumor-B-cell interactions promote isotype switching to an immunosuppressive IgG4 antibody response through upregulation of IL-10 in triple negative breast cancers. J Transl Med 20:112 (2022). PubMed: 35255925
- Assaf S et al. Up-regulation of ST18 in pemphigus vulgaris drives a self-amplifying p53-dependent pathomechanism resulting in decreased desmoglein 3 expression. Sci Rep 12:5958 (2022). PubMed: 35396567
- Liang YC et al. ADRM1 as a therapeutic target in hepatocellular carcinoma. Kaohsiung J Med Sci 37:47-54 (2021). PubMed: 32916039
- Chang GP et al. FABP4 facilitates inflammasome activation to induce the Treg/Th17 imbalance in preeclampsia via forming a positive feedback with IL-17A. Mol Ther Nucleic Acids 24:743-754 (2021). PubMed: 33996256
- Yenmis G et al. Metformin promotes apoptosis in primary breast cancer cells by downregulation of cyclin D1 and upregulation of P53 through an AMPK-alpha independent mechanism Turk J Med Sci 51:826-834 (2021). PubMed: 33350292