Mouse and Rabbit Specific HRP/AEC IHC Detection Kit - Micro-polymer (ab236467)
Overview
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Product name
Mouse and Rabbit Specific HRP/AEC IHC Detection Kit - Micro-polymer -
Detection method
Colorimetric -
Assay type
Micro-Polymer -
Product overview
This IHC detection kit is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC). It uses an HRP micro-polymer secondary antibody for highly sensitive detection.
HRP micro-polymer methods are more sensitive than the traditional streptavidin-biotin method, and they do not require a blocking step for endogenous biotin.
This kit is an exact copy of EXPOSE Mouse and Rabbit Specific HRP/AEC Detection IHC Kit ab93686, except that it uses the HRP micro-polymer antibody used during the validation of Abcam's antibodies in IHC. This HRP micro-polymer antibody was identified by our R&D team as giving the most sensitive and specific staining.
The kit includes ready-to-use hydrogen peroxide blocking reagent, protein blocking reagent, HRP micro-polymer conjugated goat anti-rabbit secondary antibody, and AEC chromogen. It includes an unconjugated rabbit anti-mouse antibody, which is used together with the HRP polymer anti-rabbit antibody to detect mouse primary antibodies.
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Notes
Other IHC detection products
For alternative kits, see Anti-Rabbit HRP-AEC Micro-polymer IHC Kit ab236468, and HRP-DAB micro-polymer IHC kits.
For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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Tested applications
Suitable for: IHC-Pmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 15 ml 125 ml — — — — — AEC Single Solution 1 x 15ml Goat anti-rabbit HRP Conjugate 1 x 15ml Hydrogen Peroxide Block 1 x 15ml Mouse Specifying Reagent (Complement) 1 x 15ml Protein block 1 x 15ml -
Research areas
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Relevance
Biotin-Free Polyvalent AEC detection is a biotin-free immunoenzymatic antigen detection system. AEC is 3-amino-9-ethyl-carbazole, a chromogenic substrate.
Associated products
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Immunohistochemistry reagents
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Substrate reagent
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC-P
Use at an assay dependent concentration. |
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab236467 has been referenced in 4 publications.
- Huang H et al. Piwil1 Regulates Glioma Stem Cell Maintenance and Glioblastoma Progression. Cell Rep 34:108522 (2021). PubMed: 33406417
- Pauk M et al. Iron overload in aging Bmp6-/- mice induces exocrine pancreatic injury and fibrosis due to acinar cell loss. Int J Mol Med 47:N/A (2021). PubMed: 33649802
- Vukicevic S et al. Autologous blood coagulum is a physiological carrier for BMP6 to induce new bone formation and promote posterolateral lumbar spine fusion in rabbits. J Tissue Eng Regen Med 14:147-159 (2020). PubMed: 31671243
- Gesualdi L et al. The PI3K/AKT Pathway Is Activated by HGF in NT2D1 Non-Seminoma Cells and Has a Role in the Modulation of Their Malignant Behavior. Int J Mol Sci 21:N/A (2020). PubMed: 33212946