Mouse and Rabbit Specific HRP/DAB IHC Detection Kit - Micro-polymer (ab236466)
Overview
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Product name
Mouse and Rabbit Specific HRP/DAB IHC Detection Kit - Micro-polymer
See all HRP/DAB detection kit kits -
Detection method
Colorimetric -
Assay type
Micro-Polymer -
Product overview
This IHC kit is used to detect mouse and rabbit primary antibodies in immunohistochemistry (IHC). It uses an HRP micro-polymer secondary antibody for highly sensitive detection.
HRP micro-polymer methods are more sensitive than the traditional streptavidin-biotin method, and they do not require a blocking step for endogenous biotin.
This kit is an exact copy of EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC Kit ab80436, except that it uses the HRP micro-polymer antibody used during the validation of Abcam's antibodies in IHC. This HRP micro-polymer antibody was identified by our R&D team as giving the most sensitive and specific staining.
The kit includes ready-to-use hydrogen peroxide blocking reagent, protein blocking reagent, HRP micro-polymer conjugated goat anti-rabbit secondary antibody, and DAB chromogen/substrate. It includes an unconjugated rabbit anti-mouse antibody, which is used together with the HRP polymer anti-rabbit antibody to detect mouse primary antibodies.
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Notes
The previous version of this kit, the EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC Kit, was used by researchers to stain over 150,000 slides since 2012 (based on volumes of reagent sold).
Other IHC detection products
For alternative kits, see anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236469, and HRP-AEC micro-polymer IHC kits.
For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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Tested applications
Suitable for: IHC-Pmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 15 ml 60 ml 125 ml 50x DAB Chromogen 1 x 0.5ml 1 x 2ml 1 x 4ml DAB substrate 1 x 15ml 1 x 60ml 1 x 125ml Goat anti-rabbit HRP Conjugate 1 x 15ml 1 x 60ml 1 x 125ml Hydrogen Peroxide Block 1 x 15ml 1 x 60ml 1 x 125ml Mouse Specifying Reagent (Complement) 1 x 15ml 1 x 60ml 1 x 125ml Protein block 1 x 15ml 1 x 60ml 1 x 125ml -
Research areas
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Relevance
HRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236466 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC-P
Use at an assay dependent concentration. |
Images
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Effect of doxycycline (DOX) treatment on the expression of MMP-2 and MMP-9 in the skinImage from Arash et al., Sci Rep; 10(1):7154. doi: 10.1038/s41598-020-64071-8.
Effect of doxycycline (DOX) treatment on the expression of MMP-2 and MMP-9 in the skin. (A) The upper panel shows representative MMP-2 staining of skin from CTRL and MFS mice at 12 months of age with or without DOX, and the lower panel shows bar graphs representing the expression of MMP-2 in the skin of CTRL and MFS mice at 12 weeks of age with or without DOX. (B) The upper panel shows representative MMP-9 staining of skin from CTRL and MFS mice at 12 months of age with or without DOX treatment. The lower panel shows bar graphs representing the expression of MMP-9 in the skin of CTRL and MFS mice at 12 weeks of age treated with or without DOX.
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Effect of doxycycline (DOX) treatment on the expression of MMP-2 and MMP-9Image from Arash et al., Sci Rep; 10(1):7154. doi: 10.1038/s41598-020-64071-8.Effect of doxycycline (DOX) treatment on the expression of MMP-2 and MMP-9 in the aorta.Immunohistochemistry staining images and bar graphs representing the expression of (A) MMP-2 and (B) MMP-9 in the aortic roots of CTRL and MFS mice at 12 weeks of age treated with or without DOX.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/DAB IHC Detection Kit - Micro-polymer (ab236466)ab236466 Mouse and Rabbit Specific HRP-DAB Detection IHC Kit on Human Tonsil staining CD20 -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)ab209064 at 1:2000 staining TMEM119 antibody in mouse cerebrum tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TMEM119 with ab209064 at 1/2000 dilution followed by the same HRP micro-polymer goat anti-rabbit secondary antibody used in this kit. Positive staining on glial cells in mouse cerebrum is observed. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0) before commencing with IHC staining protocol. Counter stained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)ab1220 staining human kidney sections by IHC-P using EXPOSE IHC detection kit ab80436 (this kit is identical to ab80436, except that it uses the same HRP polymer secondary antibody used by Abcam's R&D team for antibody validation). Formalin fixed paraffin embedded tissue sections were pre-treated using heat mediated antigen retrieval (using a pressure cooker) with sodium citrate buffer (pH 6) for 30 minutes. The section was incubated with ab1220, 5 µg/ml, for 1 hour at room temperature. DAB was used as the chromogen and the section was counterstained with haematoxylin and mounted with DPX.
Datasheets and documents
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SDS download
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Datasheet download
References (47)
ab236466 has been referenced in 47 publications.
- Trevelin SC et al. Disrupted Peyer's Patch Microanatomy in COVID-19 Including Germinal Centre Atrophy Independent of Local Virus. Front Immunol 13:838328 (2022). PubMed: 35251032
- Novotny T et al. The possible role of hypoxia in the affected tissue of relapsed clubfoot. Sci Rep 12:4462 (2022). PubMed: 35292718
- Efimova EV et al. Trace Amine-Associated Receptor 2 Is Expressed in the Limbic Brain Areas and Is Involved in Dopamine Regulation and Adult Neurogenesis. Front Behav Neurosci 16:847410 (2022). PubMed: 35431833
- Sadamoto S et al. Comparison Approach for Identifying Missed Invasive Fungal Infections in Formalin-Fixed, Paraffin-Embedded Autopsy Specimens. J Fungi (Basel) 8:N/A (2022). PubMed: 35448568
- Schregel J et al. Effects of oral glutamine supplementation on jejunal morphology, development, and amino acid profiles in male low birth weight suckling piglets. PLoS One 17:e0267357 (2022). PubMed: 35476806